TABLE 2.

Primers used in this studya

PrimerSequence
ACT195′-ATATACCGCGGACATTTTATGATGGAATGA-3′
ERG135′-CGACATTATTAGGGCCCTTTGAGAACAGCC-3′
ERG145′-CAATAGCCATATTGTCGACTGATCTTCTTG-3′
ERG155′-AATACTGCAGCAACTTTCTTTCGATTCAGTG-3′
ERG165′-CTAAGAGCTCGAATCCTGGTCCTATATTAGC-3′
UPC2-3A5′-AACAGAGCTCTACGTTATTCAGCTTTCC-3′
UPC2-3B5′-GCTTCATTAGCACAGTTGCCCATC-3′
UPC2-4A5′-TTATGGGCCCACAGTAACGAATCACATTTGTG-3′
UPC2-4B5′-GCATTCAATACTTGCCTTTAGTGC-3′
ERG11-f5′-TTTAGTTTCTCCAGGTTATGCTCAT-3′
ERG11-r5′-ATTAGCTTTGGCAGCAGCAGTA-3′
UPC2-f5′-TCCATCCTTGACCCCTAGTCCT-3′
UPC2-r5′-CGGCTGAGTTTTGATGTCTTGA-3′
18S-f5′-CACGACGGAGTTTCACAAGA-3′
18S-r5′-CGATGGAAGTTTGAGGCAAT-3′
  • a Restriction sites introduced into the primers are underlined. Primers UPC2-3B and UPC2-4B were used for sequencing UPC2 internal regions. The primer pairs ERG11-f and ERG11-r, UPC2-f and UPC2-r, and 18S-f and 18S-r were used for quantitative real-time PCR and yielded 135-bp, 100-bp, and 52-bp products, respectively.