TABLE 5.

Differential quantification of gene expression in clinical isolates compared to CIP 70-10

Function or geneDescriptionChange (fold)a
AYEBM4675BM4676
Efflux
    adeAMFP2.845.423.94
    adeBRND2.803.993.58
    adeCOMF2.282.282.72
    adeIMFPNSNS1.73
    adeJRNDNSNS1.83
    adeKOMFNSNSNS
    APC 41_138 d-serine/d-alanine/glycine transport protein2.08NSNS
    MFS 12_508MFSNSNS1.53
    RND 29_167RND2.10bNSNS
Outer membrane protein
    omp25Putative outer membrane protein1.72NSNS
    omp33-3633- to 36-kDa outer membrane protein−1.62NS1.84
    omp3434-kDa outer membrane protein2.22NS14.36
    ompAOuter membrane protein A−3.01−2.32NS
Housekeeping and metabolism
    bfmSSensor kinase component of a two-component regulatory system (biofilm)NSNS1.59
    cpn6060-kDa chaperonin−2.00NSNS
    csuA/BPutative pilus subunit, biofilm operonNS−1.721.84
    fusATranslation elongation factor EF-GNSNS1.68
    gltACitrate synthaseNSNS1.54
    ppaInorganic pyrophosphatase−2.73NSNS
    rpoCDNA-directed RNA polymerase beta subunit−1.97NSNS
Antibiotic resistance
    adcChromosomal cephalosporinase22.995.735.72
    blaOXA-23Class D carbapenemaseNSNS29.61
    blaTEM-1β-LactamaseNS1.61NS
    blaVEB-1Extended-spectrum β-lactamase3.51NSNS
    aac(3)-IaAminoglycoside adenylyltransferase2.62NSNS
    ant(2′′)-IaAminoglycoside nucleotidyltransferase2.51NA29.39
    aph(3′)-VIaAminoglycoside phosphotransferaseNANS4.04
    arr-2Rifampin ADP-ribosylating transferase1.76NSNS
    catIChloramphenicol acetyltransferase3.79NSNS
    cmlAChloramphenicol efflux pump1.73NSNS
    floRChloramphenicol efflux pump1.66NSNS
    sul1Sulfonamide resistance4.10NSNS
    tet(B)Tetracycline efflux pumpNS1.87NS
Heavy metal resistance
    HMDHeavy metal detoxification2.50NSNS
    merAMercuric ion reductase1.79NSNS
    merRMercury resistance operon regulatory protein1.96NSNS
Gene mobility and island
    groEL/int1Fusion GroEL/integrase2.71NSNS
    intI1Integrase2.32NSNS
    IS1999Insertion sequence5.26NSNS
    IS6100Insertion sequence1.87NSNS
    ISAba1Insertion sequenceNS1.83NS
    lspALipoprotein signal peptidase1.81NS1.56
    qacEdelta1Quaternary ammonium compound resistance protein6.19NSNS
    resolvase 1_318Resolvase2.56NSNS
    tniATransposaseNS2.991.59
    tnpMTransposase1.742.38NS
    uspAUniversal stress proteinNSNS1.50
  • a Positive and negative values correspond to genes with increased and decreased expression, respectively, in the clinical isolate in comparison with CIP 70-10. NS, not statistically significant. NA, not applicable.

  • b Statistically significant but not confirmed by qRT-PCR.