TABLE 2.

Susceptibilities and resistance mechanisms of non-carbapenemase-producing isolates with porin loss and their transconjugants

Isolate no.aSpeciesMIC (μg/ml) of drugbResistance mechanism(s)Integron
IPMMEMETPFOXTZPCTXCAZFEPCIPAMKTGCPOL
CY1K. pneumoniae321632>256812825664>32>256161SHV-11, DHA-1, ompK35/36 lossdfrA12-orfF-aadA2
CY1T0.250.0160.00864246412>25610.25SHV-11, DHA-1dfrA12-orfF-aadA2
PU3K. pneumoniae321664>256>25612812864>32160.250.5DHA-1, aac(6′)-Ib-cr, ompK35/36 loss
PU4K. pneumoniae16432>256816640.5>32>25640.5SHV-1, DHA-1, ompK35/36 loss
PU5K. pneumoniae16864>256256>256256256>32>2560.51SHV-12, DHA-1, aac(6′)-Ib-cr, ompK35/36 loss
PU8K. pneumoniae16832>2561281282564>32811SHV-11, DHA-1, aac(6′)-Ib-cr, ompK35/36 loss
SZ61K. pneumoniae16864>256>25612825616>32>25640.5TEM-1, SHV-1a, CMY-2, ompK35/36 loss
GZ66K. pneumoniae32464>256>256128>25632>32>2560.50.5SHV-11, DHA-1, ompK35/36 lossaadA2
GZ66T0.50.0320.00812811612811>2560.1250.25SHV-11, DHA-1
SX67K. pneumoniae4232>256>256>256>256256>32>2560.51SHV-2, CTX-M-14, DHA-1, qnrB6/S1, aac(6′)-Ib-cr, ompK35/36 loss
CY75K. pneumoniae32864>256>256>2562562>32211SHV-11, DHA-1, ompK35/36 loss
XJ81K. pneumoniae8264>256>256>256>2562561160.50.5TEM-1, SHV-12, DHA-1, aac(6′)-Ib, ompK35/36 lossdfrA12-orfF-aadA2
XJ81T0.250.0160.0081624640.50.125160.0640.25TEM-1, SHV-12, aac(6′)-Ib
PU105K. pneumoniae3216128>256>256>256>256128>32>256232TEM-1, SHV-12, CTX-M-14, DHA-1, qnrB10/B4-like, aac(6′)-Ib-cr, ompK35/36 loss
ZJ68K. pneumoniae486432>256>256256>2564>2560.1251CTX-M-15, ompK35/36 lossdfrA12-orfF-aadA2
ZJ68T0.50.0320.0641632>2562561280.125>2560.0640.5CTX-M-15dfrA12-orfF-aadA2
ZJ69K. pneumoniae32432>2561664128832>2560.51SHV-28, ompK35/36 loss
GZ83K. pneumoniae328128>256>256>256>25632>32>2560.51SHV-11, CTX-M-14, ompK35/36 lossaadA2
PU104K. pneumoniae2864256>256>256>256>25640.50.50.5TEM-1, SHV-11, CTX-M-15, qnrS1, ompK35/36 loss
PU104T0.250.0320.016256425664320.2520.250.25TEM-1, CTX-M-15
PU60E. coli32864>25625625625616>32320.1250.5TEM-1, CMY-2, ompC/F lossdfrA17-aadA5
PU60T0.50.0320.064128416640.50.2510.1250.25CMY-2
PU96E. coli328128>256>256>256>25664>32210.5TEM-1, CTX-M-14, CMY-2, ompC/F loss
PU101E. coli3216128>256>256>256>25664>6440.0640.5TEM-1, CTX-M-14, CMY-2, ompC loss, ompF DEc
PU101T0.250.0320.25>25632128>25610.2510.250.25CMY-2
PU102E. coli16864>256>256>25664>256>64640.250.5TEM-1, CTX-M-14, CMY-2, aac(6′)-Ib, ompC DEc, ompF lossdfrA17-aadA5
PU102T0.250.0320.25>256>25625664640.2510.250.25TEM-1, CTX-M-14, CMY-2
GX24E. cloacae4216>256>256256>25618>25612TEM-1, DHA-1, aac(6′)-Ib, ompC loss, ompF DEcdfrA12-orfF-aadA2c
PU41E. aerogenes32864>256128>256642568>25610.5CTX-M-14, DHA-1, qnrS1, ompC DEc, ompF loss
PU41T0.50.0320.032256112864162>2560.0640.25CTX-M-14, DHA-1, qnrS1
PU43E. coli81632128256>25632>256>3280.250.5TEM-1, CTX-M-14, aac(6′)-Ib, ompC/F losscmlA1 variant-like- aac(6′)-Ib
HX74E. coli481664>256>256256>256>3280.0640.5TEM-1, CTX-M-14, CTX-M-3, aac(6′)-Ib-cr, ompC/F lossdfrA17-aadA5
HX74T0.250.0640.064164>2562562560.2520.1250.25CTX-M-3
GX56E. cloacae4432>256>256>256256>256>32>25640.5ompC loss, ompF DEcdfrA12-orfF-aadA2
WH85E. aerogenes8816256>256>25632>2560.03210.0640.5TEM-1, CTX-M-3, ompC/F loss
NJ88E. cloacae1616128>256128>256>256>25616>2560.50.5CTX-M-14, ompC/F loss
NJ89E. cloacae1616128>256128>256>256>25616>2560.50.5CTX-M-14, ompC/F loss
NJ90E. cloacae1616128>256128>256>256>25616>2560.50.5CTX-M-14, ompC/F loss
NJ91E. cloacae1616128>256128>256>256>25616>2560.50.5CTX-M-14, ompC/F loss
GZ84K. pneumoniae118>256>25625625632>32160.50.5SHV-11, aac(6′)-Ib-cr, ompK35 loss
WH97K. pneumoniae224>25632128>25632110.50.5SHV-33, aac(6′)-Ib, ompK35 DE
RJ78C. freundii828>256>2562561286416812CMY-2, qnrA1, aac(6′)-Ib-cr, ompC loss
SZ93E. cloacae40.1250.064>256210.50.1250.06411>32ompC loss
C600E. coli0.250.0320.0081610.1250.50.0640.2510.0640.5
  • a E. coli C600 was the recipient; T at the end of the isolate number indicates the transconjugant.

  • b IPM, imipenem; MEM, meropenem; ETP, ertapenem; FOX, cefoxitin; TZP, piperacillin-tazobactam; CTX, cefotaxime; CAZ, ceftazidime; FEP, cefepime; CIP, ciprofloxacin; AMK, amikacin; TGC, tigecycline; POL, polymyxin B.

  • c DE, decreased expression.