TABLE 1.

C. albicans strains used in this study

StrainParentRelevant characteristics or genotypeaReference or source
Clinical isolates
    1002FLUS isolate from patient 914
    3795FLUR isolate from patient 914
    580FLUS isolate from patient 1414
    2440FLUR isolate from patient 1414
    945FLUS isolate from patient 1514
    1619FLUR isolate from patient 1514
    5044FLUS isolate from patient 2814
    5052FLUR isolate from patient 2814
    SC5314Wild-type reference strain7
Strains carrying introduced UPC2 alleles
    SCUPC2R11A and -BSC5314UPC2G648D-SAT1-FLIP/UPC2-2This study
    SCUPC2R12ASCUPC2R11AUPC2G648D-FRT/UPC2-2This study
    SCUPC2R12BSCUPC2R11BUPC2G648D-FRT/UPC2-2This study
    SCUPC2R13ASCUPC2R12AUPC2G648D-FRT/UPC2G648D-SAT1-FLIPThis study
    SCUPC2R13BSCUPC2R12BUPC2G648D-FRT/UPC2G648D-SAT1-FLIPThis study
    SCUPC2R14ASCUPC2R13AUPC2G648D-FRT/UPC2G648D-FRTThis study
    SCUPC2R14BSCUPC2R13BUPC2G648D-FRT/UPC2G648D-FRTThis study
    SCUPC2R21A and -BSC5314UPC2-SAT1-FLIP/UPC2-2This study
    SCUPC2R22ASCUPC2R21AUPC2-FRT/UPC2-2This study
    SCUPC2R22BSCUPC2R21BUPC2-FRT/UPC2-2This study
    SCUPC2R23ASCUPC2R22AUPC2-FRT/UPC2-SAT1-FLIPThis study
    SCUPC2R23BSCUPC2R22BUPC2-FRT/UPC2-SAT1-FLIPThis study
    SCUPC2R24ASCUPC2R23AUPC2-FRT/UPC2-FRTThis study
    SCUPC2R24BSCUPC2R23BUPC2-FRT/UPC2-FRTThis study
    SCUPC2R31A and -BSC5314UPC2A643T-SAT1-FLIP/UPC2-2This study
    SCUPC2R32ASCUPC2R31AUPC2A643T-FRT/UPC2-2This study
    SCUPC2R32BSCUPC2R31BUPC2A643T-FRT/UPC2-2This study
    SCUPC2R33ASCUPC2R32AUPC2A643T-FRT/UPC2A643T-SAT1-FLIPThis study
    SCUPC2R33BSCUPC2R32BUPC2A643T-FRT/UPC2A643T-SAT1-FLIPThis study
    SCUPC2R34ASCUPC2R33AUPC2A643T-FRT/UPC2A643T-FRTThis study
    SCUPC2R34BSCUPC2R33BUPC2A643T-FRT/UPC2A643T-FRTThis study
Reporter strains expressing GFP from the ERG11 promoter
    SCEG2A and -BSC5314UPC2-1/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R12EG2ASCUPC2R12AUPC2G648D-FRT/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R12EG2BSCUPC2R12BUPC2G648D-FRT/UPC2-2This study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R14EG2ASCUPC2R14AUPC2G648D-FRT/UPC2G648D-FRTThis study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R14EG2BSCUPC2R14BUPC2G648D-FRT/ UPC2G648D-FRTThis study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R22EG2ASCUPC2R22AUPC2-FRT/UPC2-2This study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R22EG2BSCUPC2R22BUPC2-FRT/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R24EG2ASCUPC2R24AUPC2-FRT/UPC2-FRTThis study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R24EG2BSCUPC2R24BUPC2-FRT/ UPC2-FRTThis study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R32EG2ASCUPC2R32AUPC2A643T-FRT/UPC2-2This study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R32EG2BSCUPC2R32BUPC2A643T-FRT/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R34EG2ASCUPC2R34AUPC2A643T-FRT/UPC2A643T-FRTThis study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R34EG2BSCUPC2R34BUPC2A643T-FRT/ UPC2A643T-FRTThis study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
  • a SAT1-FLIP denotes the SAT1 flipper cassette. The UPC2 and ERG11 alleles in strain SC5314 can be distinguished by EcoRI and HindIII restriction site polymorphisms, respectively. The UPC2 allele containing the polymorphic EcoRI site at position +1593 was arbitrarily designated UPC2-2 (see Table 4), and the ERG11 allele containing the polymorphic HindIII site at position +347 was designated ERG2-2.