TABLE 1.

C. albicans strains used in this study

StrainParentRelevant characteristics or genotypeaReference or source
Clinical isolates
    1002FLUS isolate from patient 9 14
    3795FLUR isolate from patient 9 14
    580FLUS isolate from patient 14 14
    2440FLUR isolate from patient 14 14
    945FLUS isolate from patient 15 14
    1619FLUR isolate from patient 15 14
    5044FLUS isolate from patient 28 14
    5052FLUR isolate from patient 28 14
    SC5314Wild-type reference strain 7
Strains carrying introduced UPC2 alleles
    SCUPC2R11A and -BSC5314 UPC2 G648D-SAT1-FLIP/UPC2-2This study
    SCUPC2R12ASCUPC2R11A UPC2 G648D-FRT/UPC2-2This study
    SCUPC2R12BSCUPC2R11B UPC2 G648D-FRT/UPC2-2This study
    SCUPC2R13ASCUPC2R12A UPC2 G648D-FRT/UPC2G648D-SAT1-FLIPThis study
    SCUPC2R13BSCUPC2R12B UPC2 G648D-FRT/UPC2G648D-SAT1-FLIPThis study
    SCUPC2R14ASCUPC2R13A UPC2 G648D-FRT/UPC2G648D-FRTThis study
    SCUPC2R14BSCUPC2R13B UPC2 G648D-FRT/UPC2G648D-FRTThis study
    SCUPC2R21A and -BSC5314 UPC2-SAT1-FLIP/UPC2-2This study
    SCUPC2R22ASCUPC2R21A UPC2-FRT/UPC2-2This study
    SCUPC2R22BSCUPC2R21B UPC2-FRT/UPC2-2This study
    SCUPC2R23ASCUPC2R22A UPC2-FRT/UPC2-SAT1-FLIPThis study
    SCUPC2R23BSCUPC2R22B UPC2-FRT/UPC2-SAT1-FLIPThis study
    SCUPC2R24ASCUPC2R23A UPC2-FRT/UPC2-FRTThis study
    SCUPC2R24BSCUPC2R23B UPC2-FRT/UPC2-FRTThis study
    SCUPC2R31A and -BSC5314 UPC2 A643T-SAT1-FLIP/UPC2-2This study
    SCUPC2R32ASCUPC2R31A UPC2 A643T-FRT/UPC2-2This study
    SCUPC2R32BSCUPC2R31B UPC2 A643T-FRT/UPC2-2This study
    SCUPC2R33ASCUPC2R32A UPC2 A643T-FRT/UPC2A643T-SAT1-FLIPThis study
    SCUPC2R33BSCUPC2R32B UPC2 A643T-FRT/UPC2A643T-SAT1-FLIPThis study
    SCUPC2R34ASCUPC2R33A UPC2 A643T-FRT/UPC2A643T-FRTThis study
    SCUPC2R34BSCUPC2R33B UPC2 A643T-FRT/UPC2A643T-FRTThis study
Reporter strains expressing GFP from the ERG11 promoter
    SCEG2A and -BSC5314 UPC2-1/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R12EG2ASCUPC2R12A UPC2 G648D-FRT/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R12EG2BSCUPC2R12B UPC2 G648D-FRT/UPC2-2This study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R14EG2ASCUPC2R14A UPC2 G648D-FRT/UPC2G648D-FRTThis study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R14EG2BSCUPC2R14B UPC2 G648D-FRT/ UPC2G648D-FRTThis study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R22EG2ASCUPC2R22A UPC2-FRT/UPC2-2This study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R22EG2BSCUPC2R22B UPC2-FRT/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R24EG2ASCUPC2R24A UPC2-FRT/UPC2-FRTThis study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R24EG2BSCUPC2R24B UPC2-FRT/ UPC2-FRTThis study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R32EG2ASCUPC2R32A UPC2 A643T-FRT/UPC2-2This study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
    SCUPC2R32EG2BSCUPC2R32B UPC2 A643T-FRT/UPC2-2This study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R34EG2ASCUPC2R34A UPC2 A643T-FRT/UPC2A643T-FRTThis study
ERG11-1/erg11-2::PERG11-GFP-caSAT1
    SCUPC2R34EG2BSCUPC2R34B UPC2 A643T-FRT/ UPC2A643T-FRTThis study
erg11-1::PERG11-GFP-caSAT1/ERG11-2
  • a SAT1-FLIP denotes the SAT1 flipper cassette. The UPC2 and ERG11 alleles in strain SC5314 can be distinguished by EcoRI and HindIII restriction site polymorphisms, respectively. The UPC2 allele containing the polymorphic EcoRI site at position +1593 was arbitrarily designated UPC2-2 (see Table 4), and the ERG11 allele containing the polymorphic HindIII site at position +347 was designated ERG2-2.