TABLE 4.

Polymorphisms in UPC2 alleles of different C. albicans strains

StrainAlleleNucleotide substitutionsa
SC5314 (S)1T1338C, C1392T, C1410A, C1539T
2A1203G, A1593G
1002 (S)1T1338C, C1392T, C1410A, C1539T
2A1203G, A1593G
3795 (R)1T1338C, C1392T, C1410A, C1539T
2A1203G, A1593G
580 (S)1T777A, T879C, C1326T, T1338C, C1392T, C1410A, C1539T
2A234G, A276G, T387C, T425G, T747A, T777A, T879C, A1203G, A1593G
2440 (R)1T777A, T879C, C1326T, T1338C, C1392T, C1410A, C1539T
2A234G, A276G, T387C, T425G, T747A, T777A, T879C, A1203G, A1593G
945 (S)1A1203G, A1593G
2A234G, A276G, T387C, T425G, T747G, T777A, T879C, A1203G, A1593G
1619 (R)1A1203G, A1593G
2A234G, A276G, T387C, T425G, T747G, T777A, T879C, A1203G, A1593G
5044 (S)1T777A, T879C, C1326T, T1338C, C1392T, C1410A, C1539T
2A234G, A276G, T387C, T425G, T747A, T777A, T879C, A1203G, A1593G, G1927A
5052 (R)A234G, A276G, T387C, T425G, T747A, T777A, T879C, A1203G, A1593G, G1927A
  • a orf19.391 in the Candida genome database (http://www.candidagenome.org/) was used as the reference sequence. The A1593G substitution (underlined) creates an EcoRI restriction site that was used to distinguish the two UPC2 alleles in strain SC5314. The sequences of the two UPC2 alleles of strain SC5314 were obtained from cloned copies (reference 15 and unpublished data). Nucleotide substitutions that result in amino acid exchanges in Upc2p are highlighted in bold. The T425G substitution, which was found in several UPC2 alleles from FLUS and FLUR isolates, causes the amino acid exchange I142S. The G1927A substitution results in the A643T gain-of-function mutation. Matched fluconazole-susceptible (S) and -resistant (R) isolates are grouped together.