TABLE 1.

Susceptibilities and resistance mechanisms of carbapenemase-producing isolates and their transconjugants

Isolate no.aSpeciesSitebMIC (μg/ml) of drugcPlasmid size(s) (kb)Resistance mechanism(s)Integron
IPMMEMETPFOXTZPCTXCAZFEPCIPAMKTGCPOL
ZJ18K. pneumoniaeZJ116816>256>2561286416>32>25610.5147, 6.4, 4.1, 3.4KPC-2, SHV-28, DHA-1, ompK35 loss
ZJ18T2121286481641>2560.060.25147KPC-2, DHA-1
ZJ70K. pneumoniaeZJ2>256256>256128>256>25664>25616>2560.250.542, 5.2, 2.5KPC-2, SHV-11, CTX-M-14, ompK35/36 lossdfrA12-orfF-aadA2
ZJ70T1681632>2566432320.1310.130.2542KPC-2
ZJ71K. pneumoniaeZJ216161616>2562563212816>2560.13142, 5.2, 2.5KPC-2, TEM-1, SHV-11, CTX-M-14, ompK35 lossdfrA12-orfF-aadA2
ZJ71T84432>2561632160.2510.130.542KPC-2
ZJ99K. pneumoniaeZJ1164832>2561286432>320.51190KPC-2, CTX-M-3, qnrS1, ompK35 DEd
ZJ99T1616832>25625612864220.250.2590KPC-2, CTX-M-3, qnrS1
ZJ86E. coliZJ1883264256643232>3210.250.5120, 23, 7.1, 6.4, 5.9, 5.3, 3.9, 2.9, 2.3, 1.0KPC-2, TEM-1, ompC lossdfrA17-aadA5
ZJ86T881664>2563232160.25320.250.5120KPC-2
ZJ87E. coliZJ1881664256643232>3210.250.5120, 23, 7.1, 5.9, 5.3, 3.9, 2.9, 2.3, 1.0KPC-2, TEM-1, ompC lossdfrA17-aadA5
ZJ87T881664>2563232160.2510.250.25120KPC-2
FZ47K. pneumoniaeFZ828>25664256>2561284>25611190, 110, 80IMP-8, TEM-1, SHV-11, CTX-M-14, qnrB2, aac(6′)-Ib, ompK35/36 lossdfrA12-orfF-aadA2
FZ47T414>2568128>256641>2560.060.25190IMP-8, TEM-1, CTX-M-14, qnrB2, aac(6′)-IbdfrA12-orfF-aadA2
FZ49K. pneumoniaeFZ824>256128>256>2562560.5>2560.51190, 110, 80IMP-8, TEM-1, SHV-11, CTX-M-14, qnrB2, aac(6′)-Ib, ompK35/36 lossdfrA12-orfF-aadA2
FZ49T414>2568128>256321>2560.060.25190IMP-8, TEM-1, CTX-M-14, qnrB2, aac(6′)-IbdfrA12-orfF-aadA2
GZ64K. pneumoniaeGZ116832>256>256256>256128210.50.5140, 49, 11, 5.9, 3IMP-4, ompK35/36 lossIMP-4-orfII
GZ64T2242568128256160.2510.060.5140, 49, 11, 5.9IMP-4IMP-4-orfII
WH76K. pneumoniaeWH242>25616128256320.510.5157IMP-4, TEM-1, SHV-14, CTX-M-3, qnrS1
WH76T482>25632256>25664210.060.557IMP-4, TEM-1, CTX-M-3, qnrS1
WH77K. pneumoniaeWH244>25632256256320.510.5157IMP-4, TEM-1, SHV-14, CTX-M-3, qnrS1
WH77T444>25632256>25664210.060.557IMP-4, TEM-1, CTX-M-3, qnrS1
SZ62C. freundiiSZ444>256256256>2566432>25610.548, 33, 5.5, 5.4, 4.2, 3.3, 2.8, 1.7, 1.2IMP-4, TEM-1, CMY-2, qnrS1, aac(6′)-Ib-cr
SZ62T444>25632128>25632410.130.2548IMP-4, qnrS1
SZ63C. freundiiSZ444>256>256>256>25612832>25610.5171, 48, 33, 6.8, 5.5, 5.4, 4.2, 3.3, 2.8, 1.7, 1.2IMP-4, qnrS1dfrA12-orfF-aadA2
SZ63T444>25632256>256642>2560.130.5171, 48IMP-4, qnrS1dfrA12-orfF-aadA2
SZ92E. cloacaeSZ8832>256128>256>256128>32110.555, 7, 5.9, 5.5, 4.9, 2.5, 1.1IMP-4, TEM-1, CTX-M-14, DHA-1, qnrB10/B4-like, aac(6′)-Ib, ompC lossorfx-aac6′-II, aadA1
RJ94E. cloacaeRJ8832>256256>256>25625640.510.598, 48IMP-4, TEM-1, CTX-M-3, ompC lossPSE-1
WH103E. cloacaeWH8832>256>256>256>256>25632110.5118, 51IMP-4, TEM-1, CTX-M-3, qnrS1, ompC lossdfrA12-aadA2
C600E. coli0.250.030.0081610.130.50.060.2510.060.5
  • a E. coli C600 was the recipient; T at the end of the isolate number indicates the transconjugant.

  • b FZ, The Affiliated Union Hospital of Fujian Medical University; GZ1, The First Affiliated Hospital of Zhongshan University; RJ, The Affiliated Ruijin Hospital of Shanghai Jiaotong University; SZ, Shenzhen People's Hospital; WH, The Affiliated Tongji Hospital of Huazhong University of Science and Technology; ZJ1, The First Affiliated Hospital of Zhejiang University; ZJ2, The Second Affiliated Hospital of Zhejiang University.

  • c IPM, imipenem; MEM, meropenem; ETP, ertapenem; FOX, cefoxitin; TZP, piperacillin-tazobactam; CTX, cefotaxime; CAZ, ceftazidime; FEP, cefepime; CIP, ciprofloxacin; AMK, amikacin; TGC, tigecycline; POL, polymyxin B.

  • d DE, decreased expression.