TABLE 1.

MICs for strains expressing characterized β-lactamasesb

SpeciesStrain no.Distinguishing characteristicβ-LactamaseMolecular classaFunctional groupaMIC (μg/ml)Reference or source
IPMMEMDORCAZ
E. coliATCC 25922Wild typeNDNANA0.120.030.030.25ATCC
DH5-α/pBR322β-Lactamase positiveTEM-1cA2b0.250.030.060.5New England Biolabs
OC 11732ESBLCTX-M-15A2be0.250.030.063217
OC 6697Plasmid AmpCCMY-2C10.50.060.06>1281
Klebsiella pneumoniaeOC 15263Wild type, basalSHV-1cA2b0.50.030.060.12This study
OC 4238overexpressedSHV-1A2b0.250.030.06215
OC 4094dESBLTEM-26cA2be0.50.060.12>25615
OC 5367CarbapenemaseKPC-2cA2f161683221
Klebsiella oxytocaOC 4076Wild typeK1A2b0.250.060.0612
Serratia marcescensOC 7555Wild type, basalC10.250.030.060.516
OC 7554CarbapenemaseSME-3cA2f>2561281280.516
E. cloacaeOC 4080OverexpressedAmpCC110.250.25324
P. aeruginosaATCC 27853Wild type, basalAmpCC120.50.52ATCC
OC 4352OverexpressedAmpCC120.50.53215
OC 13996CarbapenemaseIMP-1B3>256>256>256>2568
OC 7052eCarbapenemaseVIM-2eB36432326410
OC 12115CarbapenemaseSPM-1B3>256>256>256>256This study
OC 4083OxacillinaseOXA-10cD2d122>415
A. baumanniiOC 16708Wild typeNDND0.250.250.254This study
OC 11738CarbapenemaseOXA-23D2d646432>256This study
  • a Classification as in reference 3.

  • b ND, not detected; NA, not applicable; IPM, imipenem; MEM, meropenem; DOR, doripenem; CAZ, ceftazidime.

  • c Coding region for the β-lactamase was cloned from this plasmid or strain into the pET24a expression vector.

  • d OC 4094 is also expressed as a chromosomal SHV β-lactamase.

  • e Coding region for VIM-2 was cloned into the pET29a expression vector. OC 7052 also expressed as an uncharacterized pI-7.5 β-lactamase.