TABLE 3.

Drug, dye, and detergent susceptibilities of the major efflux-deficient E. coli strain AG102MB complemented with acrB SNP allele clones

Strain or cloneaMICb (μg/ml) of the following compoundc:
AMPCHLCACIPCVERYEtBrFAKANNOVOLETCATETTYL
AG102MB16240.0630.54416>1,024425681128
pSportI>1,024440.0040.5828>1,0248644116
pP1>1,0241680.54128128>512>1,024512>1,0243281,024
pP10>1,0243280.25412864>512>1,024512>1,0241681,024
pS1328>1,02416161451264>512>1,024512>1,0243216>1,024
  • a pSportI-based acrB clones expressed with 0.1 mM IPTG (final culture concentration) in AG102MB.

  • b MIC values of all compounds except bile acids are reported in micrograms per milliliter. MIC values of bile acids (cholic acid and taurocholic acid) are reported in milligrams per milliliter.

  • c Abbreviations: CA, cholic acid; CV, crystal violet; FA, fusidic acid; NOV, novobiocin; OLE, oleandomycin.