TABLE 1.

Dissociation constants of protein-ligand complexes determined by fluorescence titration

ProteinLigandKD [nM]
NGALFeIII·(norepinephrine)350.6 ± 9.3
FeIII·(epinephrine)3-a
FeIII·enterobactin0.4 ± 0.15
FeIII·bacillibactin0.52 ± 0.16
TlcFeIII·(norepinephrine)3-
FeIII·enterobactin243.3 ± 50.7
FeuAFeIII·(norepinephrine)31,600 ± 200
FeIII·bacillibactin26.5 ± 1.4
  • a -, no binding observed.