TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype and/or relevant phenotypeSource or reference
Strains
    P. mirabilis
        N2Wild type; TcrClinical isolate
        I2N2 derivative; PpmrI Tn5-mutagenized mutant; PBs KmrThis study
        dIpN2 derivative; PpmrI knockout mutant; PBs KmrThis study
        dIpcdIp containing pACYC184-PpmrI; PPmrI -complemented strain; CmrThis study
        dA10N2 derivative; rppA knockout mutant; PBs Kmr 48
        dA10cdA10 containing pGEM-T Easy-rppA; RppA-complemented strain; AmprThis study
    E. coli
        TOP10F′ mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupGInvitrogen
        S17-1 λ pirλ pir lysogen of S17-1 [thi pro hsdR hsdM+recA RP4 2-Tc::Mu-Km::Tn7 (Tpr Smr)]; permissive host able to transfer suicide plasmids requiring the Pir protein by conjugation to recipient cells 9
Plasmids
    pGEM-T EasyHigh-copy TA cloning vector; AmprPromega
    pGEM-4ZHigh-copy cloning vector; AmprPromega
    yT&A-xylEHigh-copy TA cloning vector containg xylE coding region; AmprProvided by T. C. Yang (21)
    pUT/mini-Tn5(Km)Suicide plasmid requiring Pir protein for replication and containing mini-Tn5 cassette containing Kmr gene 9
    pACYC184Low-copy cloning vector, P15A replicon; Cmr Tetr 7
    pACYC184-PpmrIpACYC184 containing intact PpmrI sequence, including its ribosome binding site (rbs); CmrThis study
    pGEM-T Easy-rppApGEM-T Easy containing intact rppA sequence, including its rbs; AmprThis study