TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant propertiesReference or source
Strains
    E. coli
        DH5αF, φ80dlacZ ΔM15 endA1 recA1 gyrA96 thi-1 hsdR17 (rK mK+) supE44 relA1 deoR Δ(lacZYA-argF) U169Invitrogen Life Technologies, Inc.
        TG90Derivative of the TG1 strain which carries the pcnB80 mutation and replicates plasmids at a low copy no.30
    B. subtilis
        168trpC227
        168 CmtrpC2; amyE::pX; CmrThis work
        ΔSPβtrpC2; ΔSPβ; sublancin 168 sensitive10
        ΔSPβ TctrpC2; ΔSPβ; amyE::pXTC; sublancin 168 sensitive; TcrThis work
        ANC1trpC2; ΔyokA; ΔyokB; ΔyokC; ΔyokD; ΔyokE; ΔyokF; ΔyokG; ΔyokH; ΔyokI; ΔyokJ; ΔyokK; ΔyokL; ΔyolA; ΔyolB; ΔyolC; ΔyolD; ΔuvrX; ΔyolF; ΔsunA; ΔsunT; ΔbdbA; ΔyolJ; ΔbdbB; KmrThis work
        ANC2trpC2; ΔyolC; ΔyolD; ΔuvrX; ΔyolF; ΔsunA; ΔsunT; ΔbdbA; ΔyolJ; ΔbdbB; KmrThis work
        ANC3trpC2; ΔyolF; ΔsunA; ΔsunT; ΔbdbA; ΔyolJ; ΔbdbB; KmrThis work
        ΔsunAyolFtrpC2; ΔyolF; ΔsunA; KmrThis work
        ΔsunAtrpC2; ΔsunA; KmrThis work
        ΔSPβ pGDL-yolFtrpC2; ΔSPβ; contains pDGL-yolF, which allows for constitutive expression and translation of yolF; KmrThis work
        ΔSPβ Tc pGDL-yolFtrpC2; ΔSPβ; amyE::pXTC; contains pDGL-yolF, which allows for constitutive expression and translation of yolF; Kmr; TcrThis work
        ΔSPβ pGDL-yolFCtrpC2; ΔSPβ; contains pDGL-yolFC which does not express yolF; KmrThis work
    S. aureus
        RN4220Restriction-deficient derivative of NCTC 8325; cured of all known prophages25
        RN4220 pGDL-yolFRN4220 that contains pDGL-yolF for constitutive expression of yolF; KmrThis work
        RN4220 EmRN4220 that contains the pMAD vector for Em resistance; EmrThis work
Plasmids
    pDG783pSB118 derivative; contains the Km resistance marker from Streptococcus faecalis; Apr; Kmr16
    pUC18Apr; ColE1; φ80lacZ; lac promoter45
    pUC-anc3-Kmr-pYFpUC18 derivative; contains the fragment anc3-Kmr-pYF in the multiple cloning site of the plasmidThis work
    pUC-pSU-Kmr-pYFpUC18 derivative; contains the fragment pSU-Kmr-pYF in the multiple cloning site of the plasmidThis work
    pCR-BluntII TOPOKmr; Zeocinr; lac promoter; ccdB lethal gene for E. coli is disrupted after blunt ligation of insertInvitrogen Life Technologies, Inc.
    pCR-yolFpCR-BluntII TOPO derivative; contains the yolF geneThis work
    pGDL48pSB118 derivative; contains the Km resistance marker from Streptococcus faecalis; Apr; Kmr33
    pGDL-yolFpGDL48 derivative; contains an expressed copy of the yolF geneThis work
    pGDL-yolFCpGDL48 derivative; contains a nonexpressed copy of the yolF geneThis work
    pXVector for the integration of genes in the amyE locus of B. subtilis; integrated genes are transcribed from the xylose-inducible xylA promoter; carries the xylR gene; Apr; Cmr22
    pXTCpX derivative in which the Cm resistance marker has been replaced with a Tc resistance marker; Apr; Tcr9
    pMADShuttle vector for E. coli and S. aureus; contains the bgaB gene; Emr; Apr1