TABLE 1.

Primers used for amplification/sequencing and cloning of plasmid p2007057

PrimerSequenceaUsed for
PACYC177 SmaI insert fw5′-CGTACTCCTGATGATGCATG-3′Seq inserted fragment
PACYC177 SmaI insert rev5′-GCGCATCAACAATATTTTCAC-3′Seq inserted fragment
P15′-GCCTTTTCAAATTGTGATTTTTC-3′Seq plasmid
P25′-CGTTTCCTGCTTCACAAAAT-3′Seq plasmid
P35′-CTGTACGTAATCGTTCGGTTTC-3′Seq plasmid
P45′-CCAGCGGTATCGAGGTAAAC-3′Seq plasmid
P55′-TTACTGGTTGTGATTTAACGGG-3′Seq plasmid
P65′-TCAGTAACGTCGAATGGCTTA-3′Seq plasmid
P75′-AGGCCGGAAGTCTCAAAAG-3′Seq plasmid
P85′-ATATCAGACAGTGTGGCACAG-3′Seq plasmid
P95′-CTGAAACGCGCTCAGG- 3′Seq plasmid
P105′-AACTTCTCACACTCCTGCTGTC-3′Seq plasmid
P115′-CTTTTGAGACTTCCGGCCT-3′Seq plasmid
P125′-CTGTGCCACACTGTCTGATAT-3′Seq plasmid
qnrD start EcoRV5′-GGGGATATCTTAAGGTTGTTCAAATTAATGTAC-3′Cloning qnrD
qnrD end SalI5′-CCCGTCGACTTTGATTAGTACCACATTGG-3′Cloning qnrD
qnrD fw5′-CGAGATCAATTTACGGGGAATA-3′Amplification of qnrD gene
qnrD rev5′-AACAAGCTGAAGCGCCTG-3′Amplification of qnrD gene
qnrA start EcoRV5′-CCCGATATCCTGATTAAAGGAAGCC-3′Cloning qnrA1
qnrA end SalI5′-GGGGGTCGACAGAGCTAATCCGGCAG-3′Cloning qnrA1
qnrS start PvuII5′-GGGCAGCTGCCTTTCAACAAGGAGTACTC-3′Cloning qnrS1
qnrS end SalI5′-CCCGTCGACAATTAGTCAGGATAAACAACA-3′Cloning qnrS1
  • a The recognition sites for the restriction enzymes are underlined in the nucleotide sequences of the primers used for cloning.