TABLE 4.

Susceptibility of C. krusei isolates and S. cerevisiae strains overexpressing different fungal ERG11 alleles or the multidrug efflux pump ABC1 from C. krusei to azoles measured in CSM (pH 7.0) by liquid microdilution assay

StrainMIC (μg/ml)a
FLCITCKTCMCZVRC
C. krusei
    B2399256 (32)0.5 (0.25)2 (0.5)2 (4)1 (0.5)
    IFO0011256 (128)1 (0.25)2 (1)4 (4)1 (1)
    89.021256 (32)1 (0.25)4 (0.5)4 (2)2 (0.5)
    89.102256 (32)2 (0.25)4 (0.5)4 (2)2 (0.5)
    89.221256 (32)1 (0.125)2 (0.5)4 (2)1 (0.5)
    90.147256 (128)1 (0.25)4 (1)4 (4)2 (1)
S. cerevisiae
    ADΔ0.50.0310.0310.00780.0078
    ADΔ/ScERG112.0 (4)0.25 (8)0.063 (2)0.031 (4)0.063 (8)
    ADΔ/CaERG11A2.0 (4)0.125 (4)0.125 (4)0.063 (8)0.031 (4)
    AD/CkERG11C4 (8)0.063 (2)0.063 (2)0.125 (16)0.063 (8)
    ADΔ/CkABC1g64 (128)4 (128)2 (64)0.5 (64)0.5 (64)
  • a The numbers in parentheses for the C. krusei strains indicate the MICs for these strains as determined by the CLSI method, while the numbers in parentheses for the S. cerevisiae strains indicate the fold increases in the MICs for recombinant S. cerevisiae strains versus the control strain ADΔ.