TABLE 2.

Genes up- and downregulated in C. albicans in response to FLC, AMD, and both drugs in combination

GeneCategoryUp- and downregulation (fold) inductiona in strain:
36082 (WT)1002 (WT)3795 [erg11(F136L/K134R)]610 (WT)611 (overexpression CDR1)
FAF+AFAF+AFAF+AFAF+AFAF+A
MID1Ca2+ pumps, channels, and transporters0.81.30.70.82.41.70.94.21.90.97.24.61.14.73.1
CCH1Ca2+ pumps, channels, and transporters1.37.61.81.12815.41.15325.20.79.24.61.19.33.9
PMC1Ca2+ pumps, channels, and transporters1.5282.31.26520.91.0121.628.60.935.97.90.9257.4
CRZ1Calmodulin-calcineurin pathway1.245.22.62.413.14.01.126.13.90.85.63.12.011.61.7
CMD1Calmodulin-calcineurin pathway1.30.91.30.82.71.80.83.32.60.83.52.01.27.11.6
FKS2Cell wall1.12.60.61.02.31.21.04.62.20.71.22.01.30.60.7
TUS1Cell wall1.94.52.01.04.83.01.318.98.50.82.01.71.43.13.1
SKN1Cell wall1.53.70.70.72.41.01.22.31.41.05.43.21.66.03.0
WSC2Cell wall1.33.80.60.72.51.91.02.71.50.96.63.30.83.92.8
RIM101Alkaline pH and cation overload response1.020.82.71.128.46.81.131.88.11.213.710.61.016.76.8
GYP7GTPase activity, vesicle transport1.05.11.70.928.413.21.272.825.00.922.511.01.022.811.3
TAC1bTranscription factor activity1.335.96.21.344.223.81.243.336.70.836.018.11.25.43.6
ZCF39Transcription factor activity1.34.31.41.228.311.41.353.417.41.114.68.31.011.25.1
RLM1cTranscription factor activity1.20.50.81.13.00.71.24.91.31.13.21.81.23.21.2
CLB2Cell cycle0.90.60.31.10.40.41.10.50.51.00.50.50.90.40.7
ACE2Cell cycle1.01.80.31.02.22.21.23.23.01.03.63.41.13.21.9
CLN1Cell cycle1.11.30.51.10.00.40.80.00.71.31.11.51.10.31.4
HSP90Heat shock protein. molecular chaperone0.70.31.01.20.32.51.10.52.80.80.62.11.20.81.5
FOX2Oxidation of fatty acids1.14.41.31.113.77.91.232.018.80.86.917.21.22.97.1
ERG1Ergosterol biosynthesis2.20.91.61.60.50.61.20.81.21.41.81.31.20.61.1
ERG11Ergosterol biosynthesis2.41.31.62.70.71.11.20.71.51.30.51.31.70.31.1
ERG3Ergosterol biosynthesis1.81.20.92.81.11.22.11.81.32.01.21.01.80.60.8
ERG6Ergosterol biosynthesis2.40.81.03.30.51.11.80.61.90.90.80.91.40.70.8
NRG1Morphogenesis and hyphal formation1.02.60.71.43.70.81.22.81.40.83.31.61.43.72.6
TUP1Morphogenesis and hyphal formation0.81.40.80.60.70.71.33.22.51.01.01.71.12.12.7
RFG1Morphogenesis and hyphal formation0.80.40.50.90.10.91.10.20.30.70.40.61.40.40.6
UME6Morphogenesis and hyphal formation0.70.00.50.70.00.10.60.00.20.90.50.71.10.10.2
  • a The data represent the arithmetic mean of three repetitions. Numbers in boldface represent upregulated genes (>2-fold). Numbers in italics represent downregulated genes (>2-fold). F, fluconazole; A, amiodarone.

  • b Related to azole resistance by transcriptional activation of the CDR genes (8).

  • c Related to stress response (37).