TABLE 4.

MICs of streptovaricin (STV) and rifampin (RIF) against S. spectabilis NRRL2494 and S. albus transconjugants containing different mutagenized and nonmutagenized β- and β′-subunits

StrainPlasmidAmino acid substitution(s)STV MIC (μg/ml)RIF MIC (μg/ml)
S. spectabilis NRRL 2494>200>40
S. albus J1074705
S. albus(pEM4T)pEM4T705
S. albuspEM4T-Aβ705
S. albuspEM4T-Sβ20040
S. albus Sβ′pEM4T-Sβ′705
S. albus CASβpEM4T-CASβ20040
S. albus Sβ100pEM4T-Sβ100I467L20040
S. albus Sβ101pEM4T-Sβ101N474S2005
S. albus Sβ102pEM4T-Sβ102S475A20040
S. albus Sβ103pEM4T-Sβ103T638K20020
S. albus Sβ104pEM4T-Sβ104A639S20040
S. albus Sβ105pEM4T-Sβ105N474S/S475A705
S. albus Aβ204pEM4T-Aβ204L463I705
S. albus Aβ205pEM4T-Aβ205S470N805
S. albus Aβ206pEM4T-Aβ206A471S805
S. albus Aβ207pEM4T-Aβ207S470N/A471S20040