TABLE 1.

PCR primers used for real-time RT-PCR and to sequence regulatory genes

Analysis method and genePrimerDNA sequenceAmplicon size (bp)Reference
RT-PCR
    rpoDFor5′-CGCAACAGCAATCTCGTCTGAAA-3′130This study
Rev5′-GCGGATGATGTCTTCCACCTGTT-3′This study
    ampCFor5′-GGTGCAGAAGGACCAGGCACAGAT-3′97This study
Rev5′-CGATGCTCGGGTTGGAATAGAGGC-3′This study
    oprDFor5′-CGGCGACATCAGCAACACC-3′194This study
Rev5′-GGGCCGTTGAAGTCGGAGTA-3′This study
    mexAFor5′-GGCGACAACGCGGCGAAGG-3′203This study
Rev5′-CCTTCTGCTTGACGCCTTCCTGC-3′This study
    mexCFor5′-GCAATAGGAAGGATCGGGGCGTTGG-3′102This study
Rev5′-CCTCCACCGGCAACACCATTTCG-3′This study
    mexEFor5′-TCATCCCACTTCTCCTGGCGCTACC-3′150This study
Rev5′-CGTCCCACTCGTTCAGCGGTTGTTCGATG-3′This study
    mexXFor5′-AATCGAGGGACACCCATGCACATCC-3′82This study
Rev5′-CCCAGCAGGAATAGGGCGACCAG-3′This study
Regulatory gene sequencing
    mexRFor5′-TGTTCTTAAATATCCTCAAGCGG-3′729 2
Rev5′-GTTGCATAGCGTTGTCCTCA-3′ 2
Int15′-GCGCAACCCCAGCGACCA-3′This study
    nalCFor5′-TCAACCCTAACGAGAAACGCT-3′813 2
Rev5′-TCCACCTCACCGAACTGC-3′ 2
Int 15′-CCGGCGATCGGCAAGTCC-3′This study
Int 25′-GATCCCCGCTGCCAGAGCC-3′This study
    nalDFor5′-GCGGCTAAAATCGGTACACT-3′788 2
Rev5′-ACGTCCAGGTGGATCTTGG-3′ 2