TABLE 3.

Effect of NaCl concentration on binding constants for voriconazole with CYP164A2a

[NaCl]b (M)K d (μM)ΔAmax
02.74 ± 0.470.0053 ± 0.0002
0.058.36 ± 0.620.0112 ± 0.0003
0.117.91 ± 2.790.0143 ± 0.0011
0.220.05 ± 3.770.0187 ± 0.0018
0.334.04 ± 9.660.0218 ± 0.0041
0.519.58 ± 2.070.0219 ± 0.0012
1.09.85 ± 2.990.0091 ± 0.0012
2.013.17 ± 1.370.0073 ± 0.0004
  • a The binding constants Kd and ΔAmax were determined using the Michaelis-Menten-Henri equation. Fitting the data using the Hill equation gave nHapp values between 0.8 and 1.2, indicating that the binding of voriconazole to CYP164A2 was not allosteric.

  • b The NaCl concentration in 50 mM sodium phosphate, pH 7.5, and 25% (wt/vol) glycerol.