TABLE 1.

Identification of conserved regions in mycobacterial CYP164A1 homologsa

BlockNo. of residuesBlock consensus sequence
A5526 ANRADPYPLYAXFRXXGPLQLPEANLXVFSXFXDCDEVLRHPXSXSDRXKSTVAQ
B5284 AAGAXPRPFGPPGFLFLDPPDHTRLRRLVSKAFVPRVIKALEPEIVSLVDXL
C33142 GEFDXIXDLAYPLPVAVICRLLGVPLEDEPQFS
D35201 RXXAGXWLRDYLRELIXXRRXXPGDDLXSGLIAVE
E39241 LTEDEIVATCNLLLVAGHETTVNLIANAALAMLRXPGQW
F45280 AALAADPXRAXAVXEETLRYDPPVQLVSRIAAXDMTIGGVTVPKG
G30341 FDRPDTFDPDRXXLRHLGFGKGAHFCLGAP
H44371 LARLEAXVALSAVTARFPXARLAGEPVYKPNVTLRGMSXLSVAX
  • a The online program BLOCKMAKER (http://blocks.fhcrc.org/blocks/make_blocks.html ) was used to identify conserved ungapped MOTIF regions of amino acid residues between M. smegmatis CYP164A2 and nine CYP164A1 homologs from different mycobacterial species. The mycobacterial CYPs compared were M. smegmatis CYP164A2 (accession number A0R5U2), M. leprae CYP (Q9CBE7), M. gilvum (A4T681), Mycobacterium sp. strain JLS (A3Q7G4), Mycobacterium sp. strain MCS (Q1B233), Mycobacterium sp. strain KMS (A1UN16), M. vanbaalenii (A1TGP4), M. paratuberculosis (Q744J7), M. avium (A0Q9Q7), and M. marinum (MM5268). Amino acid residues conserved between all 10 sequences are underlined. Nonconserved amino acid residues, present in 4 or fewer of the 10 CYP proteins, are represented by the letter X. The residue numbers of each block relate to the start position in the M. smegmatis CYP164A2 protein.