TABLE 1.

Mutations found in the cyp51A gene of A. fumigatus and azole susceptibility phenotypes

Phenotype and no. of strainsMIC (range)a (μg/ml)Mutation(s) in cyp51A geneSource(s) (no.) of isolate(s)
ITZVRCPOS
ITZ+ (total n = 130)
    1>160.250.25G54EbClinical
    1>160.25>16G54WbClinical
    1>168>16G138CbClinical
    1>1622M220RbClinical
    2>1610.5M220IbClinical
    1>1610.5M220VbClinical
    1>162>16M220KbClinical
    1>16>161F46Y, G89G, M172V, N248T, D255E, L358L, E427K, C454CClinical
    1216->161-40.063-0.25TR,c L98H, S297T, F495IClinical (11), environmental (1)
    1>1620.125TR, L98H, F495IClinical
    1>1640.5TR, L98H, S52TEnvironmental
    1>1680.25TR, L98H, Q88HClinical
    1>1680.5TR, L98H, N125IClinical
    1>1640.5TR, L98H, Q141HEnvironmental
    1>1640.5TR, L98H, A284AClinical
    1>1681TR, L98H, L339LClinical
    864->162-160.25-1TR, L98HClinical
    168->160.25-40.125->16NoneClinical (13), environmental (3)
ITZ (total n = 76)
    10.1250.50.016F46Y, G89GClinical
    10.250.250.063F46Y, G89G, E427K, C454CClinical
    10.2520.063F46Y, G89G, L358L, E427K, C454CClinical
    10.2510.125F46Y, G89G, M172V, L358L, C454CEnvironmental
    10.12510.063F46Y, M172V, L358L, E427K, C454CClinical
    50.125-0.50.5-10.063-0.125F46Y, G89G, M172V, L358L, E427K, C454CClinical (4), environmental (1)
    10.2510.063F46Y, G89G, M172V, N248T, D255E, E427KClinical
    20.250.250.063F46Y, G89G, M172V, N248T, D255E, L358L, E427K, C454CClinical
    10.50.50.125E130DClinical
    10.2510.5L252LClinical
    1120.25S400IClinical
    600.1250.50.031NoneClinical (47), environmental (13)
  • a Each isolate was tested once. When multiple isolates contain the same mutation, the MIC range is given. VRC, voriconazole; POS, posaconazole.

  • b Mutation known from the literature to be correlated with azole resistance.

  • c TR; tandem repeat of 34 bp in the promoter region of the cyp51A gene.