TABLE 1.

Characteristics of the P. aeruginosa isolates from Puerto Rico

StrainSample siteHospitalaHospital wardbPFGE groupMIC (μg/ml)cPCR resultdpISequence analysiseExpression off:OprD productiong
P/TCAZIPMMEMIMPVIMKPCOXAmexAoprD
PAO14110.51.01.0+
PS1Sputum1General9C82168NegNegNeg4.20.008
PS2Urine1ICU10B41286416PosNegNegPos8.0IMP-18, OXA-2
PS3Sputum2ICU1284168NegNegNeg1.00.002
PS4Sputum3General9B64>128>6464PosNegNegNeg5.4, 7.5, ≥8.5IMP-18
PS5Sputum1ICU7A>25664>64>64NegNegPosNeg6.7, ≥8.5KPC-21.80.005
PS6ASputum4ICU11A>25632>64>64NegNegPos1.80.0005
PS7AUrine4ICU11D>25664>64>64NegNegPos
PS9Sputum1General3B648816NegNegNeg
PS10Blood1General1A>25664>64>64NegNegPosNeg6.7, ≥8.5KPC-21.50.003
PS11ASputum1ICU7A>25632>64>64NegNegPos
PS12Sputum1General7B>25664>6432NegNegPos
PS13Sputum1ICU11E>25664>64>64NegNegPos
PS14Sputum1ICU10C4646416PosNegNeg
PS15ABlood1General10A81283216PosNegNeg
PS17BBlood1ICU11D>25664>64>64NegNegPosNeg6.7, ≥8.5KPC-22.00.0004
PS18ACSF1ICU11D>25632>64>64NegNegPos
PS19AUrine1General11C>25664>64>64NegNegPos
PS20ASkin5General11D>25664>64>64NegNegPos1.90.0003
PS21Skin4General11F>25664>64>64NegNegPos
PS22Urine4General3A25616816NegNegNeg
PS23Blood1General4A>256128>64>64NegNegPos6.7, ≥8.5KPC-2
PS25Sputum1General1A>25664>64>64NegNegPos
PS26Sputum3ICU3A1288816NegNegNegPos7.5, 8.0OXA-2
PS28Skin5General6128128>64>64NegNegPos7.65, ≥8.5KPC-5
PS29Sputum4General11D>25664>64>64NegNegPos
PS30Sputum4General11D>25664>64>64NegNegPos
PS31Skin4General11B>25664>64>64NegNegPos
PS33Blood1General9A32>1286432PosNegNeg
PS34Blood1ICU4A>25664>64>64NegNegPos
PS35Urine4General11A>25664>64>64NegNegPos
PS36Urine4General11B>25664>64>64NegNegPos
PS37Sputum4General11D>25664>64>64NegNegPos
PS39Urine1General8128>128>6464PosNegNegNeg5.4, 6.0, 7.5, ≥8.5IMP-184.10.015
PS40Urine6General512832>64>64NegNegPosPos6.7, 8.0, ≥8.5KPC-2, OXA-2
PS41Urine4General11D>25664>64>64NegNegPos
PS43Skin1ICU2256161632NegNegPosPos6.7, 8.0, ≥8.5KPC-2, OXA-21.80.56
PS44BloodERNR1332>1286432PosNegNegNeg5.4, 6.0, 7.5, ≥8.5IMP-183.30.36
  • a ER, emergency room.

  • b ICU, intensive care unit (includes medical, surgical, pediatric, and neurosurgical units); NR, not reported.

  • c P/T, piperacillin-tazobactam; CAZ, ceftazidime; IPM, imipenem; MEM, meropenem.

  • d Neg, negative for amplification; Pos, positive for amplification.

  • e The blaKPC and blaIMP-18 sequence represents the entire structural gene. The blaOXA-2 sequence was obtained from 555 bp of the amplified fragment.

  • f Expression data are presented as fold differences compared to the wild-type strain PAO1.

  • g OprD production was assessed by outer membrane analysis.