TABLE 2.

Antimicrobial susceptibility profiles of E. coli XL1-Blue(pLBII-VIM-2), producing WT VIM-2 MBL, and isogenic derivatives producing the A64W, W87A, and W87F mutantsa

AntibioticMIC (μg/ml)
VIM-2A64WW87FW87ApBC-SK
Ampicillin6464822
Piperacillin880.50.50.5
Cephalothin256256161616
Cefoxitin128128888
Cefuroxime256256888
Cefotaxime>32>320.120.120.12
Ceftazidime220.120.120.12
Cefepime0.50.50.060.120.06
Ceftriaxone440.120.120.12
Imipenem110.120.120.12
Meropenem0.50.5≤0.03≤0.03≤0.03
Ertapenem0.50.5≤0.03≤0.03≤0.03
Aztreonam0.120.120.120.120.12
  • a The strain carrying the empty vector (pBC-SK) is also shown for comparison.