TABLE 5.

RT-qPCR analysis of the expression of selected genes in the parR mutant and WT cells grown in BM2-glucose minimal medium with or without indolicidin peptide

GeneFold change (mean ± SD) in gene expressiona
WT/parR mutantWT with indolicidin/WT without indolicidinparR mutant with indolicidin/parR mutant without indolicidin
With indolicidinWithout indolicidin
PA155916.46 ± 6.13NTbNTNT
PA1797114.8 ± 50.011.56 ± 0.7025.3 ± 5.80.38 ± 0.26
PA1799 (parR)0.66 ± 0.08NT1.05 ± 0.015NT
PA3552 (arnB)16.34 ± 7.32NTNTNT
PA4661 (pagL)5.26 ± 0.531.33 ± 0.444.06 ± 0.641.00 ± 0.20
PA477423.34 ± 14.66NTNTNT
PA4777 (pmrB)4.88 ± 1.861.13 ± 0.502.27 ± 0.390.54 ± 0.24
PA47828.73 ± 1.92NTNTNT
  • a Fold change in gene expression comparing gene expression in the WT to gene expression in the parR mutant (WT/parR mutant) in the absence and presence of 4 μg/ml indolicidin or comparing gene expression in the presence and absence of 4 μg/ml indolicidin in the WT or parR mutant.

  • b NT, not tested.