TABLE 2.

Proteins down- or upregulated with farnesola

Protein regulation and amount of farnesol (μM)Protein spotProtein (gene)FunctionMWpINo. of peptide matches% Protein score/ confidence interval (%)NCBI nr database accession no.
Downregulation
    402aPutative stress response protein (DDR48)Damage response226024.271110068483600
2bTranslational elongation factor 1β (TEF1)Protein synthesis24942674.2531002494267
5Riboflavin synthase; hypothetical Ca019-11507 (RIB5)Involved in riboflavin synthesis30917.35.8810068467869
6Phosphoglycerate mutase; hypothetical CaO19.8522 (GPM1-2)Glycolysis; changes with drug exposure27437.55.791510068469783
7G-beta-like protein; hypothetical Ca019-9606 (ASC1)Unknown23622.16.3410068487301
8Similar to mammalian aldo/keto reductase; hypothetical CaO19.6757 (GRE3)Involved in stress response33095.26.17710068472117
9Glyceraldehyde-3-phosphate dehydrogenase (TDH1)Glycolysis; changes with drug exposure35926.76.611510068472227
10Enolase; hypothetical Ca019-8025 (ENO)Glycolysis47202.55.541310068488457
    20017ATP synthase subunit D; hypothetical CaO19.10301 (ATP7)Mitochondrial; catalyzes ATP synthesis19364.16.19810068488805
18NADH:quinone oxidoreductase; hypothetical CaO19.11095 (CQR2)Enzyme complex of the respiratory chain21714.26.51510068483141
19Heat shock protein 60 (HSP60)Mitochondrial; involved in proteins imported into the mitochondrion60378.65.221610068485963
20Orthologous to carboxymethylenebutenolidase; hypothetical CaO19.2966 (YDL086W)Hydrolase enzyme28828.45.84410068468813
22Glyceraldehyde-3-phosphate dehydrogenase (TDH1-3)Glycolysis35926.76.611810068472227
24Dihydroxy acid dehydratase (ILV3)Mitochondrion; involved in branched amino acid synthesis63462.96.21210068467901
26Pyruvate kinase (PYK1)Glycolysis55757.76.542310068482226
27S-adenosylmethionine synthase; hypothetical CaO19.8272 (SAM2)Methionine metabolism42465.85.641610068484437
Upregulation
    4023Putative mitochondrial aconitate hydratase (ACO1)Tricarboxylic acid cycle84632.75.962410068479387
88Coproporphyrinogen III oxidase (HEM13)Mitochondrion; involved in heme biosynthesis37089.45.661510068490312
89Branched chain amino acid transaminase; hypothetical CaO19.6994 (BAT1)Involved in branched amino acid synthesis408945.891110068482781
90aAlcohol dehydrogenase; hypothetical CaO19.3997 (ADH3)Functions in formaldehyde metabolism46499.68.261910068468132
91Catalase A (CTA1)Reactive oxygen metabolism54945.26.181610068474218
926-Phosphogluconate dehydrogenase; hypothetical CaO19.12491 (GND1)Plays a critical role in protecting cells from oxidative stress57164.16.141910068467359
93Pyruvate decarboxylase (CDC19/PYK1)Glycolysis627505.391810068480872
96Heat shock protein 70; hypothetical CaO19.12447 (SSA1)Molecular chaperone; involved in response to environmental stress704455.062410068467277
97Alanine/arginine aminopeptidase; hypothetical CaO19.12664 (APE2)Protein degradation107584.45.633710068491573
98Heat shock protein 90 homologue; hypothetical CaO19.6515 (HSP90)Molecular chaperone; involved in response to environmental stress80773.24.812810068469132
99aAryl-alcohol dehydrogenase; hypothetical CaO19.1048 (CAD4)Involved in drug resistance39539.76.84161006325169
    20057Heat shock protein 90 (HSP90)Molecular chaperone; involved in response to environmental stress23899.44.3316368475757
58Putative Rho protein GDP dissociation factor (RDI1)Involved in reorganization of actin cytoskeleton22947.95.15710068465635
59Putative mitochondrial aconitate dehydrogenase (ACO1)Required for tricarboxylic acid cycle and mitochondrial genome maintenance84632.75.962810068479387
60Heat shock protein 60 (HSP60)Mitochondria; oxidative stress response60378.65.2215068485963
61Aldose reductase (GRE3)Induced by oxidative stress42602.97.051710068470494
62Coproporphyrinogen III oxidase (HEM13)Mitochondria; involved in heme biosynthesis37089.45.661010068490312
66GTP binding protein; homologue to human Ranp (GSP2)Involved in the maintenance of nuclear organization, RNA processing25148.76.2261006324759
67Manganese superoxide dismutaseInvolved in defense against ROS14782.78.0424660760051
69Mitochondrial 2-enoyl thioester reductaseRequired for respiration and maintenance of mitochondrial function38645.36.361399.9968486065
70Putative aspartate aminotransferase (AAT21)Aspartate metabolism49035.38.611710068482311
72Glutathione reductase (GLR1)Oxidative stress57190.17.181010055294642
76Acetyl-CoA hydrolase/transferase (ACH1)Involved in acetyl-CoA pathway58172.56.42898.5368482646
78Phosphoglycerate mutase; hypothetical CaO19.8669 (GPM1)Glycolysis30678.17.19599.9868484809
80Outer mitochondrial membrane porin; hypothetical CaO19.1042 (POR1)Required for the maintenance of mitochondrial osmotic stability and membrane permeability29748.58.51210068484582
81NADH-cytochrome b5 reductase (MCR1)Involved in resistance against oxidative stress33555.68.5414868490698
  • a Proteins identified by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and MALDI-TOF MS as being differentially expressed in cells exposed to increasing concentrations of farnesol and unexposed cells. Acetyl-CoA, acetyl coenzyme A.