TABLE 2.

Substrate kinetics of E. cuniculi PAOa

SubstrateVmax (μM min−1)Km (μM)
N1-acetylspermine10.030
BW-17.62
N1-acetylspermine + BW-117.515
  • a Kinetic constants were obtained from Hanes-Woolf plots of various substrate concentrations for N1-acetylspermine (100 to 2,000 μM) and BW-1 (1 to 25 μM). The values for the N1-acetylspermine plus BW-1 mixture were determined from Hanes-Woolf plots of various N1-acetylspermine (100 to 2,000 μM) concentrations with fixed increasing concentrations of BW-1 (5 to 100 μM).