TABLE 2.

Kinetic parameters of the purified IMP-1 and VIM-2 enzymes

CompoundIMP-1VIM-2
kcat (s−1)Km or Ki (μM)kcat/Km (μM−1 s−1)kcat (s−1)Km or Ki (μM)kcat/Km (μM−1 s−1)
Imipenem63.5 ± 1.235.1 ± 3.81.898.9 ± 0.78.5 ± 0.512
MeropenemNDaND0.28.5 ± 0.39.4 ± 0.90.9
Biapenem44.7 ± 4.7167 ± 210.312.4 ± 0.324.7 ± 0.50.5
ME1071b0.41 ± 0.1120 ± 3.8
  • a ND, not determined.

  • b Ki value.