TABLE 1.

Sterol composition of C. albicans sterol-WT (CA14) and erg11 erg5 double-mutant (CA108) isolates following azole treatment

SterolContent (%)a
Sterol-WTerg11 erg5 double mutant
CA14CA14 FCA14 VCA14 CCA14 ICA14 KCA108CA108 FCA108 VCA108 CCA108ICA108 K
Zymosterol16.01.4
4,4-Dimethylcholesta-8,14,24-trienol3.4
Ergosterol86.845.547.330.033.036.4
14α-Methylfecosterol9.412.7
4,14α-Dimethylcholesta-8,24-dienol5.57.2
Ergosta 5,7-dienol82.457.127.442.625.024.4
Episterol1.6
14α-Methylergosta-8,24(28)-dien-3β,6α-diol25.428.635.038.037.210.432.850.045.1
Lanosterol/obtusifoliolb13.225.024.135.029.026.425.729.516.416.720.3
Eburicol4.10.99.48.28.310.2
  • a F, fluconazole; V, voriconazole; C, clotrimazole; I, itraconazole; K, ketoconazole.

  • b 14α-Methylated sterols with identical molecular weights and retention times.