TABLE 1.

MICs determined in 7H9 broth by serial dilution method after overexpressing the INH-NAD binding proteins from M. tuberculosis H37Rv in M. smegmatis

Overexpressed proteinMIC (μg/ml)a
INHETH
None (mc24517 parental strain)4.79.4
Rv1484 (InhA)300150
Rv2763c (DfrA)4.79.4
Rv3248c (SahH)4.79.4
Rv0753c (MmsA)4.79.4
Rv1187 (RocA)4.79.4
Rv0155 (PntAA)4.79.4
Rv2623 (universal stress protein)4.79.4
Rv1996 (universal stress protein)4.79.4
Rv0468 (FadB2)4.79.4
Rv2691 (CeoB/TrkA)4.79.4
Rv0091 (Mtn/Sah)NANA
Rv2858c (AldC)4.79.4
Rv1059 (unknown)4.79.4
Rv0926c (unknown)4.79.4
Rv3777 (probable oxidoreductase)4.79.4
Rv2971 (probable oxidoreductase)NANA
Rv2766 (FabG5)4.79.4
Rv2671 (RibD)4.79.4
  • a NA, not available (overexpression was not successful).