TABLE 3.

Sequencing profiles of ompK35 and ompK36

Carbapenem resistance category and isolatefMIC (μg/ml) of:OmpK35OmpK36
IPMMEMETPSequence accession no.aModificationbSequence accession no.Modification(s)
Low
    ART2008226<1<0.25<0.5FJ577672cFJ577673Thr192 del
    ART2008142<148FJ577672cFJ577673
    AIS081058142AJ011501BAH64431
    AIS070654412FJ577672cFJ577673
    AIS081072228FJ577672cFJ577673
    AIS080884248AJ011501Glu132LysBAH64431Ser255Thr
    ART2008140428FJ577672cFJ577673
    AIS070446444FJ577672cFJ577673
    HIP10924444ADG27478ACI07318Asp91Asn, Tyr198Phe, Leu311Ile
    ART2008028488AJ011501FJ577675Gly136 and Asp137 del, Ala305Pro
    ART20081374816FJ577672cFJ577673
Intermediate
    HIP144748816FJ577672cFJ577673
    ART20081368816FJ577672cFJ577673
High
    ART200802281632FJ577672cFJ577673
    ART2008139321616FJ577672cFJ577673
    ART200814381664FJ577672cFJ577673
    AIS080949321664FJ577672cFJ577673
    HIP14358321664FJ577672cFJ577673
    AIS080792323264FJ577672cFJ577673
    ART20081383232128FJ577672cNAdNDe
    ART20080243264128AJ011501BAH64431135 and 136 ins Asp
    ART20080266464256FJ577672cFJ577673
    AIS08104264128256FJ577672cFJ577673135 and 136 ins GlyAsp
    AIS080571128128256FJ577672cNAND
    ART2008141256256256FJ577672cFJ577673Thr261fsX
    ART20081355122561,024FJ577672cFJ577673Thr126fsX
    ART20080275125121,024FJ577672cFJ577673Glu78fsX
  • a Accession numbers indicate most similar reference sequences in GenBank.

  • b Predicted translational modifications, based on nucleotide sequencing data, that deviate from the sequence of the listed accession number. —, no modification; del, deletion; ins, insertion of; fsX, frameshift resulting in a premature stop codon.

  • c Previously described sequence with a frameshift that ultimately results in a stop codon after the amino acid 88 codon.

  • d NA, unable to generate a quality PCR product for sequencing despite multiple attempts.

  • e ND, not determined.

  • f Listing of isolates is as for Table 2.