TABLE 1.

Sequences of primers and probes utilized for blaKPCgene dosage studies and analysis of Tn4401 deletions (located between the istB and blaKPC genes)

Primer or probeSequence (5′-3′) (reference)
KPC forward primerGGC CGC CGT GCA ATA C
KPC reverse primerGCC GCC CAA CTC CTT CA
KPC probe (FAM)aTG ATA ACG CCG CCG CCA ATT TGT
RPOB forward primerCTG ATG CCT CAG GAT ATG ATC AAC
RPOB reverse primerCTG GCT GGA ACC AAA GAA CTC T
RPOB probe (Cy5)aCA AGC CGA TTT CCG CAG CAG TGA
Tn4401 forward primerTGA CCC TGA GCG GCG AAA GC (17)
Tn4401 reverse primerCAC AGC GGC AGC AAG AAA GC
  • a KPC probes were labeled with 6-carboxyfluorescein (FAM), and RPOB probes were labeled with Cy5 on their 5′ ends. Each contained a black hole quencher (BHQ) on its 3′ end.