TABLE 1.

Comparative analysis of the MICs of S. aureus RN4220 and the S. aureus RN4220 transformants carrying the plasmids pKKS825, pKKS2187, and pSTS7

Bacterial strainResistance genesMIC (μg/ml)a
VIR M1TIAVALCLIPIRLINERYTETKANNEOTMP
S. aureus RN422040.060.03≤0.120.2510.250.064≤10.5
S. aureus RN4220(pKKS825)aadD, tet(L), dfrK, vga(C)32≥128≥12824640.25643264≥512
S. aureus RN4220(pKKS2187)tet(L), dfrK40.060.03≤0.120.2510.25644≤1≥512
S. aureus RN4220(pSTS7)aadD, tet(L)40.060.03≤0.120.2510.256432640.5
  • a Antimicrobial agents are abbreviated as follows: VIR M1, virginiamycin M1; TIA, tiamulin; VAL, valnemulin; CLI, clindamycin; PIR, pirlimycin; LIN, lincomycin; ERY, erythromycin; TET, tetracycline; KAN, kanamycin; NEO, neomycin; and TMP, trimethoprim.