TABLE 3.

Presence of Cj0299 (blaOXA-61), β-lactamase production, and susceptibility to β-lactams in representative isolates of C. jejuni and C. coli

Campylobacter isolateablabBLAcpIdMIC(μg/ml) of β-lactame
AMPAMXAMCPENTICCARPIPTZPCLXCXMFOXCTXMEMIPMATM
C. jejuni isolates
    P270f+441821651216128323220.080.0364
    P270 blaOXA-61::cat0.50.2522484128643220.0080.03
    P83520.250.5448>256>16256642564<0.0150.06128
    P850321464128256>256>825612825680.030.06>32
    P1962220.58<4<16>256>162566425610.0150.0154
    P285++6464812864256>256>1625612825680.0150.015>128
    P286++6464812864256>256>1625612825680.250.25>128
    P305++8.964648128128>256>256-2566412840.060.06>128
    P316++8.76432812864128>256>162566412840.120.06>128
    P338++8.96464812864128>256>1612812851280.120.06128
    P338 blaOXA-61::cat2116488812812851280.0150.03
    P790++8.7646416128128256128>16>256646440.120.12>128
    P1093++12864322563225612825651212812880.50.12
    P1093 blaOXA-61::cat20.58816163225612812840.030.12
    P1618++646426464128>51232128256256160.0080.01564
    P1650++64644646464>5123225612812840.0080.06128
    P1730++646486432128>5121625612825640.0150.01532
    P1698+1281281<86432>512161286412840.0150.06128
    P1699+64644<812864256<82566412840.0150.03<1
    P843+9.23264464128<16>256>16>2566425640.030.06>128
    P854+9.21281284256128>256>256>825612812840.030.064
C. coli isolates
    P321++8.61286486464256>256>16256646440.120.12>128
    P1627+wk +32848643251216256646440.030.12>128
    P1826++128128812832128512162566412820.060.0332
  • a The isolates for which the DNA sequence of Cj0299 (blaOXA-61) was determined are shown in boldface type.

  • b +, bla gene detected by PCR; −, bla gene not detected by PCR.

  • c +, β-lactamase (BLA) positive reaction with nitrocefin; −, β-lactamase negative reaction with nitrocefin; wk +, weakly positive reaction.

  • d The pI or isoelectric point was determined by isoelectric focusing.

  • e Abbreviations: AMP, ampicillin; AMX, amoxicillin; AMC, co-amoxiclav (amoxicillin-clavulanic acid); PEN, benzylpenicillin; TIC, ticarcillin; CAR, carbenicillin; PIP, piperacillin; TZP, tazocin (piperacillin-tazobactam); CLX, cloxacillin; CXM, cefuroxime; FOX, cefoxitin; CTX, cefotaxime; MEM, meropenem; IPM, imipenem; ATM, aztreonam. The boldface MICs indicate the agents for which lower MICs were obtained for blaOXA-61-inactivated mutants.

  • f P270, strain NCTC 11168.