TABLE 1.

In vitro whole-cell susceptibility (MIC) to and 1,3-β-d-glucan synthase inhibition profiles (IC50) of echinocandin drugs for the strains included in the study

StrainGenotypePhenotypeMICcIC50d
ANFCSFMCF
FKS1FKS2Fks1paFks2pbNo serum50% SerumNo serum50% SerumNo serum50% SerumANFCSFMCF
90030WTWTWTWT0.060.500.060.120.030.251.911.920.71
218WTWTWTWT0.060.500.120.250.121.001.253.110.56
3168WTWTWTWT0.030.500.060.120.060.501.581.791.29
42997T1874CWTF625SWT2.008.008.008.000.504.00110.6697.8126.3
5847T1885CWTS629PWT4.008.008.008.002.008.003855.44987.5854.5
21900A1895GWTD632GWT1.008.004.0016.000.508.00101.8756.3343.4
3169T1896GWTD632EWT2.008.002.528.002.528.00151.5844.5444.7
42971G1894TA4129TD632YR1377STOP4.008.008.008.001.008.002543.55248.5413.4
31498WTDel1974-1976WTF659del2.008.002.008.004.008.00160117.2123.5
234WTT1975GWTF659V1.008.004.008.001.008.0086.6115.3113.5
41026WTT1976CWTF659S4.0016.0016.0016.004.0016.00858.11064.01067.0
3830WTT1987CWTS663P1.008.0016.0016.001.008.004003.55115.52569
41400WTA1997GWTD666G2.008.004.008.000.124.00175.8506.6100.6
42031WTC1998AWTD666E1.004.004.008.000.254.00127.4257.988.7
51916WTC1999AWTP667T1.598.002.008.000.404.0099.5166.2110.9
5416WTG4125AWTW1375L0.254.004.008.000.252.0085.2608.450.7
  • a WT, wild type at hot spots. Fks1p hot spot number one includes amino acids between 625 and 633 (625-FLILSLRDP-633).

  • b WT, wild type at hot spots. Fks2p hot spot number one includes amino acids between 659 and 667 (659-FLILSLRDP-667). Fks2p hot spot number two comprises amino acids between 1374 and 1381 (WT, 1374- DWVRRYTL-1381).

  • c Geometric mean values in μg ml−1 (three repetitions on three separate days).

  • d Arithmetic mean (three repetitions on three separate days) IC50s obtained using trapped 1,3-β-d-glucan synthase enzyme expressed in ng ml−1.