TABLE 1.

Deletions of S. cerevisiae genes conferring reduced fitness (higher sensitivity) or increased fitness (resistance), to MUC7 12-mer peptide, identified in the fitness screen

Open reading frameGeneLog2 intensity ratioaP valuebFunction, component of a complex, or GO termc
24 h48 h
Reduced fitness
    YPL065WVPS283.924.870.003639ESCRT I
    YPL002CVPS222.984.790.000369ESCRT II
    YJR102CVPS253.724.590.007009ESCRT II
    YCL008CVPS233.924.570.008344ESCRT I
    YGL045WRIM83.684.520.009784RIM101 pathway
    YMR077CVPS203.614.490.010161ESCRT III
    YLR025WSNF73.264.470.010608ESCRT III
    YOR030WDFG163.754.330.006508RIM101 pathway
    YNL294CRIM213.524.330.010459RIM101 pathway
    YLR417WVPS362.994.330.008441ESCRT II
    YOR275CRIM203.834.300.003918RIM101 pathway
    YGR122WYGR122W3.124.030.003781RIM101 pathway
    YHL027WRIM1013.283.990.006259RIM101 pathway
    YCR081WSRB82.973.990.028961Transcription regulation
    YOR035CSHE42.143.830.012647Actin cytoskeleton organization
    YCR068WATG151.263.750.003086Autophagy
    YBR291CCTP11.273.690.015299Mitochondrial citrate transport
    YNR052CPOP22.483.650.007818mRNA deadenylation
    YNL147WLSM71.763.520.000894mRNA catabolic process
    YIL148WRPL40A1.713.520.02152960S ribosomal subunit-ubiquitin fusion
    YHR021CRPS27B2.333.490.01084940S ribosomal subunit component
    YGL250WRMR12.343.430.004995Meiotic gene conversion and recombination
    YCR045CYCR045C1.493.400.029685Unknown
    YCR087C-ALUG11.283.390.006636Unknown
    YCR050CYCR050C2.053.370.038273Unknown
    YNL025CSSN83.263.350.025936Transcription regulation
    YDR414CERD11.923.200.008131Protein retention in ER lumen
    YCR036WRBK11.763.180.040463Putative ribokinase
    YMR154CRIM132.433.090.078834RIM101 pathway
    YHL009CYAP30.643.000.037838Transcription regulation
    YBR290WBSD21.332.900.007485Metal ion transport, protein targeting to vacuole
    YCL001W-AYCL001W-A1.002.790.015056Unknown
    YDR069CDOA41.162.790.048266ESCRT III
    YML097CVPS92.282.740.027094Protein targeting to vacuole
    YMR063WRIM92.962.680.062849RIM101 pathway
    YOL004WSIN32.002.640.021773Histone deacylation
    YDR323CPEP70.812.640.022957Vacuolar protein sorting
    YLR423CATG171.482.580.030774Autophagy
    YDR443CSSN21.322.490.000173Transcription regulation
    YBR283CSSH11.532.450.030825Cotranslational protein targeting to membrane
    YBR298CMAL312.542.410.027856Maltose transporter
    YCR024C-APMP10.842.360.020819Cation transport
    YLR330WCHS51.282.230.022982Golgi to plasma membrane transport
    YNL183CNPR10.692.220.001061Regulation of nitrogen utilization
    YNR051CBRE50.542.200.017038Protein deubiquitination
    YPL265WDIP51.722.150.047433Amino acid transport
    YPR101WSNT3091.032.130.044431Nuclear mRNA splicing via spliceosome
Increased fitness
    YBL101CECM21−0.12−2.680.021139Ubiquitin-dependent endocytosis
    YJR056CYJR056C0.13−2.020.004089Unknown
    YDR276CPMP3−0.67−1.800.020970Regulation of membrane potential
    YDR516CEMI20.01−1.730.040832Transcription regulation
    YBL089WAVT50.02−1.620.019372Amino acid vacuolar transport
    YDR067COCA6−0.34−1.580.024253Unknown
    YIL125WKGD1−0.13−1.470.006007Tricarboxylic acid cycle
    YDR346CSVF1−0.09−1.430.029858Response to oxidative stress
    YJR009CTDH20.00−1.420.028543Glyceraldehyde-3-phosphate dehydrogenase
    YDR310CSUM1−0.38−1.410.031673Transcription regulation, DNA replication initiation
    YJR014WTMA22−0.06−1.230.030385Unknown, associates with ribosome
    YJL110CGZF30.04−1.200.008931Transcription regulation, nitrogen utilization
    YMR109WMYO5−0.32−1.110.008158Type I myosin
  • a Log2 hybridization intensity ratio between untreated and treated samples collected at the indicated time point.

  • b For 48 h time point only.

  • c Saccharomyces genome database (www.yeastgenome.org).