TABLE 1.

Strains used in this study

StrainGenotype and/or descriptionReference or source
S. avermitilis ATCC 31267 18, 27
S. coelicolor
    M600Prototroph, SCP1 SCP2 1
    B725M600 trpRS1::apr; indolmycin sensitive 20
    B730B725 trpRS2(L9F), spontaneous mutant selected at 1 μg/ml indolmycin (parent of B737) 36
    B731B725, spontaneous mutant selected at 1 μg/ml indolmycin (unknown mutation[s], parent of B734)This study
    B732B725, spontaneous mutant selected at 1 μg/ml indolmycin (unknown mutation[s], parent of B735)This study
    B733B725, spontaneous mutant selected at 1 μg/ml indolmycin (unknown mutation[s], parent of B736)This study
    B734B725 TrpRS2(H48N), spontaneous mutant selected at 10 μg/ml indolmycinThis study
    B735B725 TrpRS2(H48Q), spontaneous mutant selected at 10 μg/ml indolmycinThis study
    B736B725 TrpRS2[G(−13)T], spontaneous mutant selected at 10 μg/ml indolmycinThis study
    B737B725 TrpRS2[C(−17)A/L9F], spontaneous mutant selected at 10 μg/ml indolmycinThis study
    B738B725 pJS310This study
    B739B725 pJS311This study
    B740B725 pJS312This study
    B741B725 pJS313This study
    B742B725 pJS314This study
    B743B725 pJS315This study
    B744B725 pJS316This study
    B745B725 pJS317This study
    B746B725 pJS318This study
    B747B725 pJS319This study
    B748B725 pJS320This study
    B749B725 pJS321This study
    B750B725 pJS322This study
    B751B725 pJS323This study
    B752B725 pJS324This study
    B753B725 pJS325This study
S. griseus
    NBRC 13350 26
    ATCC 12648 28
S. lividans TK24
S. venezuelae ATCC 10712
E. coli
    DH5αF φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK mK) phoA supE44 thi-1 gyrA96 relA1 λInvitrogen
    ET12567 dam dcm hsdS cat tet 36