TABLE 2.

Summary of kinetic properties of the C. glabrata 1,3-β-d-glucan synthase complexes included in the study

StrainGenotypePhenotypeVmax (nmol min−1)cK m (mM)c
FKS1FKS2Fks1paFks2pb
90030WTWTWTWT7.04 ± 0.810.13 ± 0.02
218WTWTWTWT6.85 ± 0.520.12 ± 0.04
3168WTWTWTWT6.67 ± 0.180.10 ± 0.07
42997T1874CWTF625SWT1.83 ± 0.590.17 ± 0.11
5847T1885CWTS629PWT1.44 ± 0.950.15 ± 0.05
21900A1895GWTD632GWT1.55 ± 0.410.10 ± 0.05
3169T1896GWTD632EWT1.57 ± 0.380.07 ± 0.05
42971G1894TA4129TD632YR1377STOP1.26 ± 0.240.06 ± 0.01
31498WTDel1975-1977WTF659del2.75 ± 0.220.10 ± 0.04
234WTT1975GWTF659V2.87 ± 0.850.17 ± 0.04
41026WTT1976CWTF659S3.05 ± 0.510.08 ± 0.01
3830WTT1987CWTS663P4.45 ± 0.730.16 ± 0.05
41400WTA1997GWTD666G5.05 ± 0.410.11 ± 0.03
42031WTC1998AWTD666E1.85 ± 0.440.08 ± 0.01
51916WTC1999AWTP667T0.33 ± 0.050.04 ± 0.02
5416WTG4125AWTW1375L2.71 ± 0.090.04 ± 0.01
  • a WT, wild type at hot spots. Fks1p hot spot number one includes amino acids between 625 and 633 (625-FLILSLRDP-633).

  • b WT, wild type at hot spots. Fks2p hot spot number one includes amino acids between 659 and 667 (659-FLILSLRDP-667).

  • c Arithmetic mean ± standard deviation (three repetitions).