TABLE 2.

Plasmids used in this study

PlasmidDescriptionVector backboneReference or source
pUZ8002RP4 derivative, OriT, Kanr 36
pGemTpUC-derived, lacZ′, AmprPromega
pBluescript KS+pUC ori AmprAgilent Technologies (Stratagene)
pMS81 oriT, ΦBT1 attB-int, HygrpSET152 10
pIJ10257 oriT, ΦBT1 attB-int, Hygr, ermEp*pMS81 12
pJS310 ermEp*-SCO3334 ORF (trpRS1)pIJ10257This study
pJS311 ermEp*-SAV4725 ORFpIJ10257This study
pJS312 ermEp*-SGR3809 ORFpIJ10257This study
pJS313 S. coelicolor TrpRS2(WT) locuspMS81This study
pJS314 S. coelicolor TrpRS2[G(−13)T] locuspMS81This study
pJS315 S. coelicolor TrpRS2[G(−13)T/L9F] locuspMS81This study
pJS316 S. coelicolor TrpRS2 [G(−13)T/H48Q] locuspMS81This study
pJS317 S. coelicolor TrpRS2[C(−17)A] locuspMS81This study
pJS318 S. coelicolor TrpRS2[C(−17)A/L9F] locuspMS81This study
pJS319 S. coelicolor TrpRS2[C(−17)A/H48Q] locuspMS81This study
pJS320 ermEp*-S. coelicolor TrpRS2(L9F) ORFpIJ10257This study
pJS321 ermEp*-S. coelicolor TrpRS2(H48Q) ORFpIJ10257This study
pJS322 ermEp*-S. coelicolor TrpRS2(L9F/H48Q) ORFpIJ10257This study
pJS323 ermEp*-S. coelicolor TrpRS2(Q13K) ORFpIJ10257This study
pJS324 ermEp*-S. coelicolor TrpRS2(L9F/Q13K) ORFpIJ10257This study
pJS325 ermEp*-S. coelicolor TrpRS2(H48N) ORFpIJ10257This study
pJS326SGI trpRS2 full-length cDNA from indolmycin producerpGemTThis study
pJS327SGI3809cDNA fragment from indolmycin producerpGemTThis study
pJS328 S. coelicolor TrpRS2(WT) ORFpBluescript KS+This study
pJS329 S. coelicolor TrpRS2(L9F) ORFpBluescript KS+This study
pJS330 S. coelicolor TrpRS2[G(−13)T] locuspBluescript KS+This study