TABLE 2.

MICs of pyrazolones on WT M. tuberculosis H37Rv in the presence of efflux inhibitors verapamil, reserpine, and CCCP

CompoundMIC, μg/ml (resistance ratio)a
7H9 broth7H9 + verapamil7H9 + reserpine7H9 + CCCP
AZI-33510.25 (4)0.5 (2)0.5 (2)
AZI-3360.50.25 (2)0.25 (2)0.25 (2)
AZI-37721 (2)0.25 (8)1 (2)
AZI-21925664 (4)Not tested128 (2)
AZI-4106416 (4)6416 (4)
AZI-4086416 (4)32 (2)16 (4)
AZI-352>168 (>2)8 (>2)8 (>2)
INH0.030.030.030.03
  • a MICs were performed as described in Materials and Methods by resazurin-based microplate assay. Verapamil and reserpine were used at 50-μg/ml and CCCP was added at 0.5-μg/ml concentrations. The resistance ratio (given in parentheses) is defined as MIC7H9/MIC7H9 + respective inhibitor. Each reported value is from an average of at least two independent assay results.