Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
M. tuberculosis H37Rv ATCC 27294Virulent strain of M. tuberculosisATCC
pMV261Mycobacterial expression vector28
pBAN0192936 bp of intact Rv1218c cloned into the PvuII-HindIII sites of pMV261This study
pAZI0290Derived from pGOAL19 (20) by deleting the lacZ gene as a BamHI-BamHI fragmentLab stock
pBAN0366Truncated Rv1218c cloned into pAZI0290This study