TABLE 2.

Steady-state kinetic constants of NDM-1, IMP-1, and VIM-2

CompoundNDM-1IMP-1aVIM-2b
Km (μM)kcat (s−1)kcat/Km (s−1/μM)Km (μM)kcat (s−1)kcat/Km (s−1/μM)Km (μM)kcat (s−1)kcat/Km (s−1/μM)
Penicillin G16110.685203200.6249561.14
Ampicillin22150.662009504.8DNA
Piperacillin12141.17NDcNDND72330.45
Cephalothin1040.4021482.444571.28
Cefoxitin4910.0281622430.12
Cefotaxime1060.5841.30.3532280.86
Cefuroxime850.613780.2222120.55
Ceftazidime18150.034480.1898890.90
AztreonamND>1,000>0.01<0.0001ND<0.5ND
Cefepime77130.171170.6618450.03
Imipenem94200.2139461.210100.99
Meropenem49120.2510500.12510.28
Clavulanic acidNDNRdNR
  • a From Spencer et al. (32).

  • b From Poirel et al. (23).

  • c ND, not detected.

  • d NR, not reported.