TABLE 3.

DNA sequence analysis of phoQ and pmrB from polymyxin B-resistant clinical isolates of P. aeruginosa

IsolateAmino acid alteration for:
PhoQaPmrBb
NY214Nucleotide substitution (T at position 1779141 to G) resulting in amino acid substitution V260GNo change
NY21517 nucleotides deletion from position 1278818 generated a frameshift with a truncated protein at the 152nd amino acidNo change
NY217Nucleotide substitution (A at position 1279029 to G) resulting in amino acid substitution H223RNo change
NY219Nucleotide substitution (T at position 1779141 to G) resulting in amino acid substitutionNo change
NY220No changeNucleotide substitution (G at position 5365488 to A) resulting in amino acid substitution A247T; nucleotide substitution (T at position 5365792 to C) resulting in amino acid substitution Y345H
  • a The sequence of full-length phoQ (1,347 bp; 448 amino acids) was compared to that of P. aeruginosa PAO1 (www.pseudomonas.com).

  • b A putative histidine box (H-box; 550 bp) as designated by Moskowitz et al. (20) was analyzed for NY214, NY215, NY217, and NY219; the full-length sequence of pmrB (1,434 bp; 477 amino acids) was analyzed for NY220.