TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or descriptionSource or reference
E. coli XL1endA1 hsdR17 (hsdR hsdM+) supE44 thi-1 recA1 gyrA96 relA1 D(lac) [F′ proAB lacIqZDM15 Tn10 (Tetr)]Stratagene
S. cerevisiae KLN1MATa ERG1::URA3 leu2 ura3 trp114
Plasmids
    pRS315Centromere yeast/E. coli shuttle vectorK. Kuchler
    pBIG12.3-kb PstI fragment with the wild-type ERG1 gene in pBluescript15
    pNS12.3-kb PstI fragment with the wild-type ERG1 gene in pRS31515
    pG25SpRS315 with 2.3-kb PstI fragment with allelic erg1G25S gene generated by site-directed mutagenesisThis study
    pG27SpRS315 with 2.3-kb PstI fragment with allelic erg1G27S gene generated by site-directed mutagenesisThis study
    pG30SpRS315 with 2.3-kb PstI fragment with allelic erg1G30S gene generated by random mutagenic PCRThis study
    pL37PpRS315 with 2.3-kb PstI fragment with allelic erg1L37P gene generated by random mutagenic PCRThis study
    pR269GpRS315 with 2.3-kb PstI fragment with allelic erg1R269G gene generated by random mutagenic PCRThis study
    pD209ApRS315 with 2.3-kb PstI fragment with allelic erg1D209A gene generated by site-directed mutagenesisThis study
    pG210ApRS315 with 2.3-kb PstI fragment with allelic erg1G210A gene generated by site-directed mutagenesisThis study
    pG334ApRS315 with 2.3-kb PstI fragment with allelic erg1G334A gene generated by site-directed mutagenesisThis study
    pD335ApRS315 with 2.3-kb PstI fragment with allelic erg1D335A gene generated by site-directed mutagenesisThis study
    pD335WpRS315 with 2.3-kb PstI fragment with allelic erg1D335W gene generated by site-directed mutagenesisThis study
    pD335FpRS315 with 2.3-kb PstI fragment with allelic erg1D335F gene generated by site-directed mutagenesisThis study
    pD335PpRS315 with 2.3-kb PstI fragment with allelic erg1D335P gene generated by site-directed mutagenesisThis study