TABLE 1.

MICs and MBCs of AMPs and enrofloxacin for M. pulmonis MpUR1.1a

DrugMIC (μM)/ (μg/ml)MIC/MIC EFXbMBC (μM)/ (μg/ml)MBC/MBC EFXb
Alamethicin6.25/12.315.625/49.131.2
Globomycin0.4/0.26112.5/8.215.6
Gramicidin S3.12/3.67.86.25/7.17.8
Surfactin25/25.962.5100/103.6125
EFX0.4/0.1410.8/0.291
  • a MIC determinations were realized in Hayflick medium containing 5% horse serum with the standard concentration (106 CFU/ml) of exponential-growth-phase cells of MpUR1.1 For MBC determinations, 0.1-ml aliquots were withdrawn after 2 h for AMPs and 24 h for enrofloxacin and then diluted and plated for viable-cell count. Experiments were performed in triplicate.

  • b The comparison of AMP efficacy with that of EFX is indicated by using the ratio of MICs or MBCs (μM).