TABLE 1.

Bacterial strains

StrainDescriptionaSource or reference
P. aeruginosa
    PAO1Wild typeB. W. Holloway
    FE10PAO1 nuoG::Tn5-HgThis study
    FE49PAO1 galU::Tn5-HgThis study
    FE53PAO1 mexZ::Tn5-HgThis study
    FE57PAO1 rplY::Tn5-HgThis study
    FE10UFE10 galU::FRTThis study
    FE53UFE53 galU::FRTThis study
    FE57UFE57 galU::FRTThis study
    FE53GFE53 nuoG::FRTThis study
    FE57GFE57 nuoG::FRTThis study
    FE57ZFE57 mexZ::FRTThis study
    FE53GUFE53G galU::FRTThis study
    FE57GUFE57G galU::FRTThis study
    FE57UZFE57U mexZ::FRTThis study
    FE57GZFE57G mexZ::FRTThis study
    FE57GUZFE57GU spontaneous mutant with an Ala20-to-Val change in MexZThis study
    KJ7106PAO1 ΔPA5471This study
    FE60PAO1 ΔmexXYThis study
    FE63FE57 ΔPA5471This study
    FE64FE57 ΔmexXYThis study
E. coli
    DH5αsupE44 endA1 hsdR17(rK mK+) thi-1 recA1 Δ(argF-lacZYA)U169 φ 80dlacZΔM15 phoA gyrA96 relA1 deoR λGibco-BRL
    SM10thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Kanr)8
    S17.1recA thi pro hsdRM+ RP4-2-Tc::Mu Km::Tn7 (Tmpr Strr)38
    S17.1λpirrecA thi pro hsdRM+ RP4-2-Tc::Mu Km::Tn7 λpir (Tmpr Strr)29
  • a Abbreviations: Kanr, resistance to kanamycin; Strr, resistance to streptomycin; Tmpr, resistance to trimethoprim.