TABLE 2.

Primers used in this study

Function and primerNucleotide sequence (5′-3′) (endonuclease)aSource or reference
Gene expression
    uvrD1CAC GCC TCG CCC TAC AGC A20
    uvrD2GGA TCT GGA AGT TCT GCT CAG C20
    mexB1ATC CGC CAG ACC ATC GCC A15
    mexB2CAT CAC CAG GAA CAC GAG GAG G15
    mexY1aTTA CCT CCT CCA GCG GC19
    mexY1bGTG AGG CGG GCG TTG TG19
    PA5471-AAGC TAC AGC AGG TCG AGA CGThis study
    PA5471-BTTG ATG TCG AGC AGT TCC AGThis study
    nuoH1GGT CGG CTC GTT CAA CAThis study
    nuoH2TGG CGG CGA AGT AGA AGThis study
    pth-1CGT GGC TGA CCG CAA GTAThis study
    pth-2GGG CAT GTC GAG TTC GTCThis study
Cloning
    L25-1CTC TCT AGA CGA AAA CGC CCC GCT CC (XbaI)This study
    L25-2CTC TCT AGA TTG CGG TCA GCC ACG AG (XbaI)This study
Gene inactivation
    galUrecACTC GAA TTC CTT CGT CCA TGT CGA AGG AT (EcoRI)This study
    galUrecBCTC TCT AGA CGG GCA ACT ATG TGG AGT TC (XbaI)This study
    nuoGrecACTC GGA TCC ACC CAC CAG AAC CAG GAA CT (BamHI)This study
    nuoGrecBCTC AAG CTT GAG TTG CCG GAC ACT ACC AG (HindII)This study
    mexZrecACTC GGA TCC GGC GCG ACA GTA GCA TAT AA (BamHI)This study
    mexZrecBCTC AAG CTT CGT GAA GCT ACC GTG ACA GA (HindIII)This study
    mexZb-1AAG CTT AAG CTT GCG TTC GCA CTT GAG GTA GAG (HindIII)6
    mexGb-1ACG CGG ATC CGT TCT CGA CGA TCA CCC ACTC (BamHI)6
    mexHb1ACC GGA ATT CCA CCA GGA AGA ACA GCG GTAC (EcoRI)6
    mexHf1ACG CGG ATC CCT GGA TGC TGG TCT ACA CCCT (BamHI)6
    In5471A3CTC GAG CTC GAG CTG GAT TTC TTC CTC GAC (SacI)This study
    In5471A4CTC GGA TCC TCC TCG ATT ACC TCG ACC TC (BamHI)This study
Sequencing
    M13FGTA AAA CGA CGG CCA GT44
    M13RCAG GAA ACA GCT ATG AC44
    merA1CCA CGG GAC ACG GTG AAThis study
    merA2CGG CAT CGC GGT TAG ATThis study
    SeqmexZ1GCA GCC CAG CAG GAA TAG24
    SeqmexZ2GCC TGT CGG TGC TCT ACA TC24
  • a Restriction sites were introduced into primers; these are underlined in the sequences, with the corresponding endonucleases indicated in parentheses). All amplification reactions were conducted at 61°C except in sequencing experiments (50°C).