TABLE 5.

Phenotypic and genotypic characteristics of 119 E. coli CTX-M producer strains

Antimicrobial resistance patternapI(s)bGenotype by PCR (gene type by sequencing)c:PFGE profiled (no. of strains)Hospital codeTotal no. of strains
blaTEMblaCTX-MblaOXAISEcp1IS26IS903
AM A/S AT CF CT FX TX TZ XM KZ PM PI P/T TI CI NX OF AK GM TO5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0010A1
AM A/S A/C AT CF CT TX TZ XM KZ PM PI P/T TI CI NX OF GM TO7.4; 8.9+ (15)+ (30)+0010D2
AM A/S A/C AT CF CT TX TZ XM KZ PM PI P/T TI CI NX OF AK TO5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0006E1
AM A/S AT CF CT TX TZ XM KZ PM PI P/T TI CI NX OF AK GM TO5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0010C1
AM A/S AT CF CT TX TZ XM KZ PM PI P/T TI CI NX OF GM TO T/S5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0010C1
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OF AK GM TO T/S5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0010B1
AM A/S AT CF CT TX TZ XM KZ PM PI P/T TI CI NX OF GM TO5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0010C1
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OF GM TO T/S5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0009 (1),A, B, C, D, E, F, H19
0010 (13), 0012 (1),
0013 (1), 0015 (1), 0017 (1), ND (1)
5.4; 7.4; 8.9+ (1)e+ (15)+ (30)+0010C1
5.4; 8.9+ (1b)+ (32)+0019E1
5.4; 8.9+ (1b)+ (15)+0019E1
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OF GM TO8.1+ (14)+0005C1
7.4; 8.9+ (15)+ (30)+0007 (1), 0010 (3)B, D, H4
5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0016 (1), 0009 (2),A, B, C, D, E, G, H, I52
0010 (48), 0011 (1)
5.4; 7.4; 8.9+ (1a)+ (15)+ (30)+0010H1
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OF TO T/S5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0010I1
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OF GM5.4; 8.9f+ (1b)+ (15)+ (30)+0010A1
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OF TO7.4; 8.9+ (15)+ (30)+0010D, E3
5.4; 8.9+ (1b)+ (15)+0010D1
5.4; 7.4; 8.9+ (1b)+ (15)+ (30)+0006 (2), 0008 (1), 0010 (7)C, D, E, I10
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OF T/S5.4; 8.9+ (1b)+ (15)+0010A2
AM AT CF CT TX TZ XM KZ PM PI TI CI NX OF TO7.4; 8.9+ (15)+ (30)+0010E1
AM A/S AT CF CT TX TZ XM KZ PM PI TI CI NX OFg5.4; 8.9+ (1b)+ (15)+0006(1), 0010 (1)E2
AM A/S CF CT TX XM KZ PI TI CI NX OF GM TO T/S5.4; 8.1+ (1b)+ (14)++0001E1
AM A/S CF CT TX XM KZ PI TI CI NX OF T/S5.4; 8.1+ (1b)+ (14)+NDC1
AM A/S AT CF CT TX XM KZ PM PI TI T/Sg5.4; 8.9+ (1b)+ (15)+0004E1
AM A/S CF CT TX XM KZ PM PI TI T/Sg5.4; 8.1+ (1b)+ (14)+0018C1
AM A/S CF CT TX XM KZ PM PI TIg8.1+ (14)+0002 (1), 0003 (2), ND (1)C, D, I4
5.4; 8.1+ (1c)+ (14)+0014D1
AM AT CF TX XM KZ PI TIg5.4; 8.9+ (1b)+ (15)+0010E1
  • a AM, ampicillin; A/S, ampicillin-sulbactam; A/C, amoxicillin-clavulanic acid; AT, aztreonam; CF, cephalothin; FX, cefoxitin; CT, cefotaxime; TX, ceftriaxone; TZ, ceftazidime; XM, cefuroxime; KZ, cefazolin; PM, cefepime; PI, piperacillin; P/T, piperacillin-tazobactam; TI, ticarcillin; CI, ciprofloxacin; NX, norfloxacin; OF, ofloxacin; AK, amikacin; GM, gentamicin; TO, tobramycin; T/S, trimethoprim-sulfamethoxazole.

  • b pIs were determined by IEF: 5.4 corresponds to TEM-1A, TEM-1B, or TEM-1C enzyme production; 7.4 corresponds to OXA-30 production; 8.1 corresponds to CTX-M-14; and 8.9 corresponds to CTX-M-15 or CTX-M-32 enzyme production.

  • c blaTEM genes amplified by PCR were identified by sequencing as blaTEM-1A, blaTEM-1B, or blaTEM-1C; blaCTX-M genes were identified by sequencing as blaCTX-M-14, blaCTX-M-15, or blaCTX-M-32; and blaOXA genes were identified by sequencing as blaOXA-30. +, Amplification by PCR; −, no amplification by PCR.

  • d PFGE profile and number of strains with each profile, deduced from the dendrogram presented on Fig. 1. ND, not determined.

  • e More than one blaTEM-1 was present.

  • f β-Lactamase with pI 7.4, corresponding to OXA-30 enzyme production, was not detected by IEF.

  • g Non-multidrug-resistant pattern.