TABLE 1.

Primers used for site-directed mutagenesis

PrimerPrimer sequencea
rtL80I5′-CCAACTTGTCCTGG ATATCGCTGGATGTGTCTGCG-3′
rtL180M5′-CCTCAGTCCGTTTCTCATGGCTCAGTTTACTAG-3′
rRtM204I5′-GGCTTTCAGTTATATCGATGATGTGGTATTGGGGG-3′
PC5′-GCCTTGGGTGGCTTTAGGGCATGGACATCGACC-3′
  • a Nucleotide changes are in boldface and underlined.