TABLE 4.

LMV sensitivities and relative replication yields of wild-type HBV and its mutant derivativesa

HBV variantLMV EC50 (μM)bRelative replication yieldcEffect of L80I on replication efficiencyd
Without LMVWith 1 μM LMVWithout LMVWith 1 μM LMV
Wild type0.41 ± 0.191.00 ± 0.150.33 ± 0.04
rtL80I0.03 ± 0.010.57 ± 0.050.06 ± 0.0143% decrease**82% decrease**
rtM204IR0.18 ± 0.000.38 ± 0.04
rtL80I/M204IR0.39 ± 0.063.76 ± 0.43117% increase*902% increase**
rtL180M/M204IR0.19 ± 0.040.65 ± 0.08
rtL80I/L180M/M204IR0.35 ± 0.010.34 ± 0.0484% increase*48% decrease**
PC0.46 ± 0.121.08 ± 0.060.35 ± 0.04
PC/rtL80I0.03 ± 0.020.5 ± 0.060.04 ± 0.0054% decrease**88% decrease**
PC/rtM204IR0.28 ± 0.031.40 ± 0.16
PC/rtL80I/M204iR0.70 ± 0.080.27 ± 0.03150% increase**80% decrease**
PCrtL180M/M204IR0.32 ± 0.090.54 ± 0.06
PC/rtL80I/L180M/M204IR0.67 ± 0.023.27 ± 0.38109% increase**506% increase**
  • a The wild type is genotype D; PC signifies that the G1896A mutation in the PC region (which prevents HBeAg synthesis) is present.

  • b EC50s are means ± standard deviations of the pooled results from three independent assays. R, resistant (i.e., no dose-dependent inhibition of viral replication observed at LMV concentrations up to 10 μM).

  • c Relative replication yields are the means ± standard deviations from at least three independent assays.

  • d Percent changes in relative replication yield for each pair with and without LMV, with statistically significant differences of P < 0.05 (*) and P < 0.01(**) indicated.