TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionReference(s) or source
Strains
    KU80ΔA. fumigatus pyrGAF::ΔKU80; wild-type strain sensitive to echinocandin drugs4
    EMFR-S678PHomologous recombinant fks1(S678F) mutant of KU80 formed following transformation with linear pRG3-(pyr4)-AMA1-Fks1S678P cut with HpaIThis study
    EMFR-S678P/WTfks1 mutant of KU80 transformed with uncut autonomously replicating plasmid pRG3-(pyr4)-AMA1-Fks1S678P; also contains wild-type FKS1This study
    EMFR-FKS1-RestoredStrain derived from EMFR-S678P/WT after plasmid evictionThis study
Plasmids
    pRG3-AMA1Contains A. nidulans AMA1 and pyr4 genes1, 2, 16
    pRG3-AMA1-Fks1S678PContains A. fumigatus fks1(S678P) in pRG3-(pyr4)-AMA1This study