TABLE 1.

Plasmid backbone analysis of the IncHI2 plasmids in transconjugants obtained from Salmonella and Escherichia coli CTX-M-2 and CTX-M-9 producers

Plasmid (ESBL)Parental strain (source)Country (yr)HI2 replicon typePCR result for:Reference
smr10-11smr17-18smr207-208smr239-240O1R_160terFarsBsmr201smr92-93smr136tnsDIntegron-borne gene cassettes
p1639-SA-00-1 (CTX-M-2)S. enterica serovar Virchow (poultry)Belgium (2000)HI2-pAPEC-O1-R++++++dfrA1-aadA1This study
p142-SA-01-1 (CTX-M-2)S. enterica serovar Virchow (poultry)Belgium (2001)HI2-pAPEC-O1-R++++++dfrA1-aadA1This study
p03-1902-1 (CTX-M-2)S. enterica serovar Virchow (poultry product)Belgium (2003)HI2-pAPEC-O1-R++++++dfrA1-aadA1This study
p04-9275-1 (CTX-M-2)S. enterica serovar Typhimurium (human feces)French Guiana (2004)HI2-pAPEC-O1-R++++++drfA12-orfF-aadA2This study
pROU-1 (CTX-M-9)S. enterica rough (human feces)France (2002)HI2-R478+++++++++dfrA16-aadA2This study, 17
p2437-1 (CTX-M-9)S. enterica serovar Virchow (chicken feces)France (2003)HI2-R478+++++++++dfrA16-aadA2This study, 17
p2731-1 (CTX-M-9)S. enterica serovar Virchow (retail chicken meat)France (2003)HI2-R478+++++++++dfrA16-aadA2This study, 17
p3464b-1 (CTX-M-9)S. enterica serovar Virchow (chicken environment)France (2003)HI2-R478+++++++++dfrA16-aadA2This study, 17
112-D-T (CTX-M-9)S. enterica serovar Virchow (human feces)Spain (2000)HI2-R478+++++++++dfrA16-aadA28, 9
1185-D-T (CTX-M-9)E. coli (human feces)Spain (1998)HI2-R478+++++++++dfrA16-aadA28, 9
1406-D-T (CTX-M-9)E. coli (human feces)Spain (1999)HI2-R478+++++++++dfrA16-aadA28, 9
R478aHI2-R478++++++++++10
pAPEC-01-RbHI2-pAPEC-O1-R++++++aadA111
  • a Expected PCR result based on the R478 sequence (EMBL accession no. BX664015).

  • b Expected PCR result based on the pAPEC-O1-R sequence (EMBL accession no. DQ517526).